Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/115766
DC Field | Value | |
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dc.title | Identification and molecular cloning of a p21cdc42/rac1-activated serine/threonine kinase that is rapidly activated by thrombin in platelets | |
dc.contributor.author | Teo, M. | |
dc.contributor.author | Manser, E. | |
dc.contributor.author | Lim, L. | |
dc.date.accessioned | 2014-12-12T07:32:12Z | |
dc.date.available | 2014-12-12T07:32:12Z | |
dc.date.issued | 1995-11-03 | |
dc.identifier.citation | Teo, M.,Manser, E.,Lim, L. (1995-11-03). Identification and molecular cloning of a p21cdc42/rac1-activated serine/threonine kinase that is rapidly activated by thrombin in platelets. Journal of Biological Chemistry 270 (44) : 26690-26697. ScholarBank@NUS Repository. | |
dc.identifier.issn | 00219258 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/115766 | |
dc.description.abstract | The brain-enriched p21cdc42/rac1-activated serine/threonine kinase, p65PAK, was identified and purified on the basis of overlays with [γ-32P]GTP-Cdc42 onto SDS-fractionated proteins (Manser, E., Leung, T., Salihuddin, H., Zhao, Z.-S., and Lim, L. (1994) Nature 367, 40-46). In this study, the ubiquitously expressed P21cdc42/rac1 binding protein with relative molecular weight of 62,000 was purified from rat testes and shown to contain peptides related to PAK. It has thus been designated as the γ-PAK isoform (α- and β-isoforms being brain enriched). Isolation of γ-PAK cDNAs show that the kinase is highly conserved with α-PAK in both the p21 binding and kinase domains. The purified protein exhibited kinase activity that was activated by GTP-Cdc42 or GTP-Rac1 in vitro. In platelets, a p62 in situ renaturable kinase was recognized by antibodies raised against γ-PAK. This thrombin-activated protein kinase appears to coprecipitate with another kinase of Mr 86,000, suggesting that PAK may be part of a thrombin-responsive signaling complex. | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | INSTITUTE OF MOLECULAR & CELL BIOLOGY | |
dc.description.sourcetitle | Journal of Biological Chemistry | |
dc.description.volume | 270 | |
dc.description.issue | 44 | |
dc.description.page | 26690-26697 | |
dc.description.coden | JBCHA | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Staff Publications |
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