Please use this identifier to cite or link to this item: https://doi.org/10.1111/j.1365-2303.2012.01000.x
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dc.titleThe positive impact of cytological specimens for EGFR mutation testing in non-small cell lung cancer: A single South East Asian laboratory's analysis of 670 cases
dc.contributor.authorPang, B.
dc.contributor.authorMatthias, D.
dc.contributor.authorOng, C.W.
dc.contributor.authorDhewar, A.N.
dc.contributor.authorGupta, S.
dc.contributor.authorLim, G.L.
dc.contributor.authorNga, M.E.
dc.contributor.authorSeet, J.E.
dc.contributor.authorQasim, A.
dc.contributor.authorChin, T.M.
dc.contributor.authorSoo, R.
dc.contributor.authorSoong, R.
dc.contributor.authorSalto-Tellez, M.
dc.date.accessioned2014-12-12T07:14:10Z
dc.date.available2014-12-12T07:14:10Z
dc.date.issued2012-08
dc.identifier.citationPang, B., Matthias, D., Ong, C.W., Dhewar, A.N., Gupta, S., Lim, G.L., Nga, M.E., Seet, J.E., Qasim, A., Chin, T.M., Soo, R., Soong, R., Salto-Tellez, M. (2012-08). The positive impact of cytological specimens for EGFR mutation testing in non-small cell lung cancer: A single South East Asian laboratory's analysis of 670 cases. Cytopathology 23 (4) : 229-236. ScholarBank@NUS Repository. https://doi.org/10.1111/j.1365-2303.2012.01000.x
dc.identifier.issn09565507
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/115328
dc.description.abstractObjectives: To compare the rejection rates of non-small cell lung cancer (NSCLC) samples obtained by differing sampling methods for testing by Sanger sequencing for epidermal growth factor receptor (EGFR) mutations. To assess the association between unsatisfactory outcomes and the quantity of DNA extracted from cytological versus histological samples. Methods: Six hundred and seventy NSCLC samples referred to our centre from 2008 to 2010 were reviewed as a consequence of sample rejection, presence of EGFR mutations, cytological versus histological sampling methods, DNA quantity and the unsatisfactory genotyping rate. Results: Eighty samples were rejected for testing in similar proportions of histological and cytological samples (11.9% versus 10.9%) usually (n = 75) because the amount of cellular material was judged insufficient in small biopsies or cytology samples. The remaining 590 samples on which EGFR testing was attempted yielded 51 (8.6%) unsatisfactory test outcomes caused by failure of the polymerase chain reaction (PCR) (n=47 cases), uninterpretable Sanger chromatograms (n=3 cases) and insufficient DNA extracted for PCR (n=1 case). The difference in rates of unsatisfactory outcomes between cytological samples (seven of 147 samples or 4.7%) versus tissue samples (44 of 443 samples or 9.9%) was clinically relevant but not statistically significant (Mann-Whitney test; P
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1111/j.1365-2303.2012.01000.x
dc.sourceScopus
dc.subjectCytological specimens
dc.subjectEGFR mutation
dc.subjectNon-small cell lung cancer
dc.subjectSanger sequencing
dc.subjectTyrosine kinase inhibitors
dc.typeArticle
dc.contributor.departmentCANCER SCIENCE INSTITUTE OF SINGAPORE
dc.description.doi10.1111/j.1365-2303.2012.01000.x
dc.description.sourcetitleCytopathology
dc.description.volume23
dc.description.issue4
dc.description.page229-236
dc.description.codenCYTPE
dc.identifier.isiut000306508400004
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