Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/115187
Title: Micropropagation of citrus mitis blanco-multiple bud formation from shoot and root explants in the presence of 6-Benzylaminopurine
Authors: Sim, G.E. 
Goh, C.J. 
Loh, C.S. 
Keywords: 6-benzylaminopurine
bud formation from roots
Citrus mitis
micropropagation
shoot regeneration
Issue Date: 1989
Citation: Sim, G.E.,Goh, C.J.,Loh, C.S. (1989). Micropropagation of citrus mitis blanco-multiple bud formation from shoot and root explants in the presence of 6-Benzylaminopurine. Plant Science 59 (2) : 203-210. ScholarBank@NUS Repository.
Abstract: Different vegetative parts of Citrus mitis seedlings and mature plants were used as explants in culture. Shoots were readily produced from epicotyl, leaf, shoot tip and nodal segments of seedling explants. A high frequency (66-100%) of shoot regeneration was obtained from shoot tip and nodal stem segments of mature plants cultured on medium with or without benzylaminopurine (BAP). Bud induction from roots was examined in 3 systems: (a) intact roots on whole seedlings; (b) intact roots on decapitated (shootless) seedlings; (c) excised roots. The highest yield of buds was obtained from intact roots on whole seedlingx cultured in medium with 0.5 mg 1-1 BAP, from which 275 shoots were obtained from 13 cultures in 9 weeks. Regenerated shoots were rooted in basal medium and the plantlets were successfully transplanted to soil. The role of growth substances on bud formation from roots is discussed. © 1989.
Source Title: Plant Science
URI: http://scholarbank.nus.edu.sg/handle/10635/115187
ISSN: 01689452
Appears in Collections:Staff Publications

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