Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.brainres.2012.11.015
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dc.titleMetabotropic glutamate receptor 5 (mGluR5) regulates proliferation and differentiation of neuronal progenitors in the developmental hippocampus
dc.contributor.authorXiao, X.L.
dc.contributor.authorMa, D.L.
dc.contributor.authorWu, J.
dc.contributor.authorTang, F.-R.
dc.date.accessioned2014-12-12T07:12:13Z
dc.date.available2014-12-12T07:12:13Z
dc.date.issued2013-02-01
dc.identifier.citationXiao, X.L., Ma, D.L., Wu, J., Tang, F.-R. (2013-02-01). Metabotropic glutamate receptor 5 (mGluR5) regulates proliferation and differentiation of neuronal progenitors in the developmental hippocampus. Brain Research 1493 : 1-12. ScholarBank@NUS Repository. https://doi.org/10.1016/j.brainres.2012.11.015
dc.identifier.issn00068993
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/115185
dc.description.abstractMetabotropic glutamate receptor 5 (mGluR5) is involved in neural stem cell self-renewal, proliferation, differentiation and survival. In this study, we aimed to further determine the role of mGluR5 in the development of hippocampus using mGluR5 deficit (mGluR5-/-) and wild type (mGluR5+/+) mice at different developmental ages. We showed that the number of BrdU, NeuroD and DCX immunopositive cells was reduced significantly in mGluR5-/- than in mGluR5+/+ mice from postnatal 7 days (P7) to P28, but not at P60. The length and intensity of DCX immunopositive apical dendrites in the dentate gyrus of mGluR5-/- mice were much shorter and lower than in mGluR5+/+ mice respectively at P14, P21 and P28. NeuN immunostaining indicated an accelerated maturation of hippocampal neurons in mGluR5 -/- mice. When mGluR5+/+ mice were treated with 2-methyl-6-(phenylethynyl) pyridine (MPEP), a selective antagonist of mGluR5, decreased proliferation of progenitor cells was observed in the hippocampus at early postnatal developmental stages. At P14, there were more BrdU+ cells in the stratum granulosum and subgranular layer of the dentate gyrus in mGluR5+/+ than in mGluR5-/- mice, but the percentage of BrdU++NeuroD+/BrdU+ in the dentate gyrus did not change significantly between the two genotypes of mice. Western Blot study suggested that programmed neuronal death was p53-dependent apoptosis in the developmental hippocampus in mGluR5+/+ mice. © 2012 Elsevier B.V.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.brainres.2012.11.015
dc.sourceScopus
dc.subjectCell proliferation
dc.subjectDentate gyrus
dc.subjectDevelopmental hippocampus
dc.subjectMetabotropic glutamate receptor 5
dc.typeArticle
dc.contributor.departmentTEMASEK LABORATORIES
dc.description.doi10.1016/j.brainres.2012.11.015
dc.description.sourcetitleBrain Research
dc.description.volume1493
dc.description.page1-12
dc.description.codenBRREA
dc.identifier.isiut000314376700001
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