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Title: Green tea polyphenol targets the mitochondria in tumor cells inducing caspase 3-dependent apoptosis
Authors: Hsu, S.
Lewis, J.
Singh, B.
Schoenlein, P.
Osaki, T.
Athar, M.
Porter, A.G. 
Schuster, G.
Keywords: Apoptosis
Breast cancer
Oral cancer
Issue Date: Mar-2003
Citation: Hsu, S.,Lewis, J.,Singh, B.,Schoenlein, P.,Osaki, T.,Athar, M.,Porter, A.G.,Schuster, G. (2003-03). Green tea polyphenol targets the mitochondria in tumor cells inducing caspase 3-dependent apoptosis. Anticancer Research 23 (2 B) : 1533-1539. ScholarBank@NUS Repository.
Abstract: Induction of apoptosis by green tea polyphenols has been observed in various tumor cell systems, but whether green tea polyphenol-induced apoptosis requires caspase 3 for execution has not been confirmed. We previously reported that green tea polyphenol-induced apoptosis involved Apaf-1 accumulation and caspase 3 activation in the cytosol. In the current study, tumor cells either with deleted caspase 3 gene or expressing wild-type caspase 3 were treated with increasing concentrations of green tea polyphenol(s), followed by morphological analysis and caspase 3 activity assay. The caspase 3 null parental cell line was further examined in comparison with a well-characterized, caspase 3 wild type oral carcinoma cell line by MTT assay and BrdU incorporation assay. The results demonstrated that, while the mitochondrial function gradually declined to insignificant levels, caspase 3 null cells did not undergo apoptosis, which suggested that green tea polyphenol-induced apoptosis is a mitochondria-targeted, caspase 3-executed mechanism.
Source Title: Anticancer Research
ISSN: 02507005
Appears in Collections:Staff Publications

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