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|Title:||Antisense telomerase induced cell growth inhibition, cell cycle arrest and telomerase activity down-regulation in gastric and colon cancer cells||Authors:||Wong, S.C.H.
Cell cycle arrest
|Issue Date:||Jan-2003||Citation:||Wong, S.C.H.,Yu, H.,Moochhala, S.M.,So, J.B.Y. (2003-01). Antisense telomerase induced cell growth inhibition, cell cycle arrest and telomerase activity down-regulation in gastric and colon cancer cells. Anticancer Research 23 (1 A) : 465-469. ScholarBank@NUS Repository.||Abstract:||Background: Antisense telomerase RNA (anti-hTR) coupled with 2′, 5′-linked tetraadenylate (2-5A) shows an inhibitory effect on cell growth and induces apoptosis in certain kinds of cancer. This experiment examined the anti-hTR effect on gastric and colorectal cancer cells. Materials and Methods: Gastric cancer cells CRL-5822, CRL-5971 and colorectal cancer cells HTB-38, CCL-247 were treated with repeated doses of anti-hTR. Cell growth and cell cycle parameters were analysed. Telomerase activity was measured by TRAP assay. Control oligonucleotides and normal fibroblast were used as control. Results: After two doses of anti-hTR, the cell viability for CRL-5822, CRL-5971, HTB-38 and CCL-247 cells was reduced to 15%, 12%, 37% and 37%, respectively. The DNA histogram showed cells arrested at the G2 / M - phase and the sub-G1 peak found indicated that apoptosis had occurred. Conclusion: Anti-hTR demonstrated inhibition of cell growth and cell cycle arrest on gastric and colorectal cancer cells through a telomerase regulation pathway. Its specificity towards cancerous tissues but not normal tissues suggested a potential for anti-cancer therapy.||Source Title:||Anticancer Research||URI:||http://scholarbank.nus.edu.sg/handle/10635/115000||ISSN:||02507005|
|Appears in Collections:||Staff Publications|
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