Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/114836
Title: VARIOUS LEVELS OF ERYTHROMYCIN ON THE DEVELOPMENT OF ANTIBIOTIC RESISTANCE IN ESCHERICHIA COLI
Authors: WANG TIANREN
Keywords: erythromycin,ecoli,antibiotic resistances,ermK,MLSB
Issue Date: 16-Apr-2014
Citation: WANG TIANREN (2014-04-16). VARIOUS LEVELS OF ERYTHROMYCIN ON THE DEVELOPMENT OF ANTIBIOTIC RESISTANCE IN ESCHERICHIA COLI. ScholarBank@NUS Repository.
Abstract: THIS STUDY EVALUATED MICROBIAL FITNESS UNDER SELECTIVE PRESSURE OF VARIOUS CONCENTRATIONS OF ERYTHROMYCIN AND THE DEVELOPMENT OF ERYTHROMYCIN RESISTANCE GENES IN AN ESCHERICHIA COLI (E. COLI) STRAIN. THE DEVELOPMENT OF ERYTHROMYCIN RESISTANCE GENES AND GENES EXPRESSIONS WERE EVALUATED WITH ONE CONVENTIONAL CULTURE-BASED METHOD, PLATE COUNTING METHOD, AND TWO MOLECULAR MICROBIOLOGY TECHNIQUES, FLUORESCENCE IN SITU HYBRIDIZATION ANALYSIS AND QUANTITATIVE POLYMERASE CHAIN REACTION. THE RESULTS INDICATED THAT E. COLI CELLS ADAPTED TO DIFFERENT ERYTHROMYCIN RESISTANCE LEVELS UNDER SELECTIVE PRESSURE IN THE PRESENCE OF ERYTHROMYCIN. FURTHERMORE, THE CONCENTRATION AT ONE MINIMUM INHIBITORY CONCENTRATION WAS MOST EFFECTIVE TO SELECT FOR ANTIBIOTIC RESISTANCE. THE BIOLOGICAL COSTS ASSOCIATED WITH FITNESS LED TO DIFFERENT REGROWTH RATES FOR DIFFERENT CONCENTRATIONS AND EVEN VIABLE BUT NONCULTURABLE CELLS DURING CULTIVATION. IN SUMMARY, THE ERYTHROMYCIN RESISTANT E. COLI STRAIN WELL ADAPTED TO TH
URI: http://scholarbank.nus.edu.sg/handle/10635/114836
Appears in Collections:Master's Theses (Open)

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