Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.neuroscience.2011.10.024
Title: SnoU6 and 5S RNAs are not reliable miRNA reference genes in neuronal differentiation
Authors: Lim, Q.E.
Zhou, L. 
Ho, Y.K.
Wan, G. 
Too, H.P.
Keywords: GeNorm
MicroRNA (miRNA)
Neuronal differentiation
Normfinder
Quantitative PCR (qPCR)
Reference genes
Issue Date: 29-Dec-2011
Citation: Lim, Q.E., Zhou, L., Ho, Y.K., Wan, G., Too, H.P. (2011-12-29). SnoU6 and 5S RNAs are not reliable miRNA reference genes in neuronal differentiation. Neuroscience 199 : 32-43. ScholarBank@NUS Repository. https://doi.org/10.1016/j.neuroscience.2011.10.024
Abstract: Accurate profiling of microRNAs (miRNAs) is an essential step for understanding the functional significance of these small RNAs in both physiological and pathological processes. Quantitative real-time PCR (qPCR) has gained acceptance as a robust and reliable transcriptomic method to profile subtle changes in miRNA levels and requires reference genes for accurate normalization of gene expression. 5S and snoU6 RNAs are commonly used as reference genes in microRNA quantification. It is currently unknown if these small RNAs are stably expressed during neuronal differentiation. Panels of miRNAs have been suggested as alternative reference genes to 5S and snoU6 in various physiological contexts. To test the hypothesis that miRNAs may serve as stable references during neuronal differentiation, the expressions of eight miRNAs, 5S and snoU6 RNAs in five differentiating neuronal cell types were analyzed using qPCR. The stabilities of the expressions were evaluated using two complementary statistical approaches (geNorm and Normfinder). Expressions of 5S and snoU6 RNAs were stable under some but not all conditions of neuronal differentiation and thus are not suitable reference genes. In contrast, a combination of three miRNAs (miR-103, miR-106b and miR-26b) allowed accurate expression normalization across different models of neuronal differentiation. © 2011 IBRO.
Source Title: Neuroscience
URI: http://scholarbank.nus.edu.sg/handle/10635/114390
ISSN: 03064522
DOI: 10.1016/j.neuroscience.2011.10.024
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

17
checked on Oct 10, 2019

WEB OF SCIENCETM
Citations

13
checked on Oct 10, 2019

Page view(s)

98
checked on Oct 11, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.