Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.gene.2007.10.027
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dc.titleDevelopment of a heat shock inducible gfp transgenic zebrafish line by using the zebrafish hsp27 promoter
dc.contributor.authorWu, Y.L.
dc.contributor.authorPan, X.
dc.contributor.authorMudumana, S.P.
dc.contributor.authorWang, H.
dc.contributor.authorKee, P.W.
dc.contributor.authorGong, Z.
dc.date.accessioned2014-12-02T06:52:37Z
dc.date.available2014-12-02T06:52:37Z
dc.date.issued2008-01-31
dc.identifier.citationWu, Y.L., Pan, X., Mudumana, S.P., Wang, H., Kee, P.W., Gong, Z. (2008-01-31). Development of a heat shock inducible gfp transgenic zebrafish line by using the zebrafish hsp27 promoter. Gene 408 (1-2) : 85-94. ScholarBank@NUS Repository. https://doi.org/10.1016/j.gene.2007.10.027
dc.identifier.issn03781119
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/114314
dc.description.abstractIn the present study, a zebrafish hsp27 promoter was isolated and used to develop heat shock inducible gfp transgenic zebrafish. The endogenous hsp27 mRNAs were constitutively expressed from 4 hpf and increased in several regions of brain, heart and somites in early embryogenesis until 24 hpf. Subsequently, the expression was reduced significantly but maintained in the heart and ears. Heat shock induced hsp27 mRNAs in the blastoderm from 6 hpf and later in somites, branchial arches and several regions of brain. Similarly in hsp27-gfp transgenic zebrafish, constitutive GFP expression was observed from 11 hpf. GFP expression was mainly in the skin cells and increased to the peak level at 7 dpf, followed by a reduction. The constitutive GFP expression in the heart was initiated from 50 hpf and maintained even in the adult fish. After heat shock, GFP expression was mainly induced in the muscle in addition to a mild increase in the skin and heart. The early stages of the embryos were more sensitive than late stages as the time required for induced GFP expression in the muscle is shorter. Thus, the hsp27-gfp transgenic line generally recapitulates the expression pattern and heat shock inducibility of endogenous hsp27 RNAs. We also tested the potential of using the hsp27-gfp transgenic zebrafish embryos for heavy metal induction and demonstrated the inducibility of GFP expression by arsenic; this pattern of induction was also supported by examination of endogenous hsp27 mRNA. © 2007 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.gene.2007.10.027
dc.sourceScopus
dc.subjectArsenic
dc.subjectEmbryo
dc.subjectHeart
dc.subjectHsp25
dc.subjectMuscle
dc.subjectSmall heat shock protein
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.departmentOFFICE OF THE DEPUTY PRESIDENT(RES&TECH)
dc.description.doi10.1016/j.gene.2007.10.027
dc.description.sourcetitleGene
dc.description.volume408
dc.description.issue1-2
dc.description.page85-94
dc.description.codenGENED
dc.identifier.isiut000253033600010
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