Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/113700
Title: Urinary trans,trans-muconic acid determined by liquid chromatography: Application in biological monitoring of benzene exposure
Authors: Lee, B.-L.
New, A.-L.
Kok, P.-W.
Ong, H.-Y.
Shi, C.-Y.
Ong, C.-N. 
Keywords: Environmental hazards
Toxicology
Vanillic acid as internal standard
Issue Date: 1993
Citation: Lee, B.-L.,New, A.-L.,Kok, P.-W.,Ong, H.-Y.,Shi, C.-Y.,Ong, C.-N. (1993). Urinary trans,trans-muconic acid determined by liquid chromatography: Application in biological monitoring of benzene exposure. Clinical Chemistry 39 (9) : 1788-1792. ScholarBank@NUS Repository.
Abstract: We describe a sensitive and specific high-performance liquid-chromatographic method for determining the benzene metabolite, trans, trans-muconic acid (ttMA) in urine by measuring ultraviolet absorbance at 265 nm. We mix 1 mL of urine sample with 2 mL of Tris buffer containing vanillic acid as internal standard (IS) and percolate this through a preconditioned ion-exchange column. After rinsing the column with phosphoric acid solution, acetate buffer, and deionized water, we elute the analytes with 2 mL of an equivolume solution of 1.5 mol/L sodium chloride and methanol. Of this, 5 μl is injected into the HPLC column. The mobile phase used consists of, per liter, 10 mL of acetic acid, 100 mL of methanol, and the rest 5 mmol/L sodium acetate. The flow rate was started at 1 mL/min and increased to 1.5 mL/min after 6 min. ttMA and IS were detected at 5.2 and 10.2 min, respectively. The lowest detection limit is 125 pg. Analytical recovery and reproducibility generally exceeded 90%. We validated the method with urine samples collected from normal persons and from refinery workers exposed to benzene concentrations
Source Title: Clinical Chemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/113700
ISSN: 00099147
Appears in Collections:Staff Publications

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