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|Title:||Urinary trans,trans-muconic acid determined by liquid chromatography: Application in biological monitoring of benzene exposure||Authors:||Lee, B.-L.
Vanillic acid as internal standard
|Issue Date:||1993||Citation:||Lee, B.-L.,New, A.-L.,Kok, P.-W.,Ong, H.-Y.,Shi, C.-Y.,Ong, C.-N. (1993). Urinary trans,trans-muconic acid determined by liquid chromatography: Application in biological monitoring of benzene exposure. Clinical Chemistry 39 (9) : 1788-1792. ScholarBank@NUS Repository.||Abstract:||We describe a sensitive and specific high-performance liquid-chromatographic method for determining the benzene metabolite, trans, trans-muconic acid (ttMA) in urine by measuring ultraviolet absorbance at 265 nm. We mix 1 mL of urine sample with 2 mL of Tris buffer containing vanillic acid as internal standard (IS) and percolate this through a preconditioned ion-exchange column. After rinsing the column with phosphoric acid solution, acetate buffer, and deionized water, we elute the analytes with 2 mL of an equivolume solution of 1.5 mol/L sodium chloride and methanol. Of this, 5 μl is injected into the HPLC column. The mobile phase used consists of, per liter, 10 mL of acetic acid, 100 mL of methanol, and the rest 5 mmol/L sodium acetate. The flow rate was started at 1 mL/min and increased to 1.5 mL/min after 6 min. ttMA and IS were detected at 5.2 and 10.2 min, respectively. The lowest detection limit is 125 pg. Analytical recovery and reproducibility generally exceeded 90%. We validated the method with urine samples collected from normal persons and from refinery workers exposed to benzene concentrations||Source Title:||Clinical Chemistry||URI:||http://scholarbank.nus.edu.sg/handle/10635/113700||ISSN:||00099147|
|Appears in Collections:||Staff Publications|
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