Please use this identifier to cite or link to this item: https://doi.org/10.1074/jbc.M207546200
DC FieldValue
dc.titleThe growth hormone-binding protein is a location-dependent cytokine receptor transcriptional enhancer
dc.contributor.authorGraichen, R.
dc.contributor.authorSandstedt, J.
dc.contributor.authorGoh, E.L.K.
dc.contributor.authorIsaksson, O.G.P.
dc.contributor.authorTörnell, J.
dc.contributor.authorLobie, P.E.
dc.date.accessioned2014-12-01T06:57:12Z
dc.date.available2014-12-01T06:57:12Z
dc.date.issued2003-02-21
dc.identifier.citationGraichen, R., Sandstedt, J., Goh, E.L.K., Isaksson, O.G.P., Törnell, J., Lobie, P.E. (2003-02-21). The growth hormone-binding protein is a location-dependent cytokine receptor transcriptional enhancer. Journal of Biological Chemistry 278 (8) : 6346-6354. ScholarBank@NUS Repository. https://doi.org/10.1074/jbc.M207546200
dc.identifier.issn00219258
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/113679
dc.description.abstractIn the rat, a growth hormone-binding protein (GHBP) exists that is derived from the growth hormone (GH) receptor gene by an alternative mRNA splicing mechanism such that the transmembrane and intracellular domains of the GH receptor are replaced by a hydrophilic carboxyl terminus. In isolation, the GHBP is inactive, although it does compete with the receptor for ligand binding in the extracellular space and therefore inhibits the cellular response to GH. The GHBP is also located intracellularly and is translocated to the nucleus upon ligand stimulation. We show here that endogenously produced GHBP, in contrast to exogenous GHBP, was able to enhance the STAT5-mediated transcriptional response to GH. Interestingly, when the GHBP was targeted constitutively to the nucleus by the addition of the nuclear localization sequence of the SV40 large T antigen, greater enhancement of STAT5. mediated transcription was obtained. The transcriptional enhancement did not require GH per se and was not specific to the GH receptor, since similar enhancement of STAT5-mediated transcription by nuclear localized GHBP was obtained with specific ligand stimulation of both prolactin and erythropoietin receptors. Thus, the GHBP exerts divergent effects on STAT5-mediated transcription depending on its cellular location. The use of a soluble cytokine receptor as a location-dependent transcriptional enhancer and the ligand-independent involvement of the extracellular domain of a polypeptide ligand receptor in intracellular signal transduction provide additional novel mechanisms of transcriptional control.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1074/jbc.M207546200
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentMEDICINE
dc.description.doi10.1074/jbc.M207546200
dc.description.sourcetitleJournal of Biological Chemistry
dc.description.volume278
dc.description.issue8
dc.description.page6346-6354
dc.description.codenJBCHA
dc.identifier.isiut000181129400108
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

23
checked on Jan 31, 2023

WEB OF SCIENCETM
Citations

20
checked on Jan 31, 2023

Page view(s)

108
checked on Feb 2, 2023

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.