Please use this identifier to cite or link to this item:
|Title:||Simultaneous Determination of Tocotrienols, Tocopherols, Retinol, and Major Carotenoids in Human Plasma||Authors:||Lee, B.-L.
|Issue Date:||Dec-2003||Citation:||Lee, B.-L., New, A.-L., Ong, C.-N. (2003-12). Simultaneous Determination of Tocotrienols, Tocopherols, Retinol, and Major Carotenoids in Human Plasma. Clinical Chemistry 49 (12) : 2056-2066. ScholarBank@NUS Repository. https://doi.org/10.1373/clinchem.2003.022681||Abstract:||Background: Epidemiologic evidence suggests that the concentrations of antioxidant vitamins in human plasma may play an important role in numerous chronic diseases, such as cancer and cardiovascular disease. However, methods for simultaneous measurement of these antioxidants are scarce. We developed and validated a new HPLC method for simultaneous determination of these vitamers in human plasma that uses a novel column-switching approach. Methods: The new method uses liquid-liquid extraction and isocratic separation with two monomeric C18 columns maintained at 35 and 4°C coupled with ultraviolet-visible and fluorometric detection. This method could separate 14 vitamers and 3 internal standards within 27 min. No additional modifier was required; the mobile phase was acetonitrile-methanol (65:35 by volume), and the flow rate was 1 mL/min. Results: For photodiode array detection, the detection limits (signal-to-noise ratio >3) were 0.02 mg/L for β-carotene, lutein, zeaxanthin, and canthaxanthin; 0.01 mg/L for all-trans-retinol, β-cryptoxanthin, α-carotene, and lycopene; and 0.1 mg/L for all tocopherols and tocotrienols. The detection limit was at least 25-fold lower (0.004 mg/L) when fluorometry was used for measurement of δ-, γ-, and α-tocotrienol and δ-tocopherol compared with ultraviolet detection. The recovery and imprecision of the assay were generally >90% and||Source Title:||Clinical Chemistry||URI:||http://scholarbank.nus.edu.sg/handle/10635/113651||ISSN:||00099147||DOI:||10.1373/clinchem.2003.022681|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Nov 26, 2022
WEB OF SCIENCETM
checked on Nov 10, 2022
checked on Nov 24, 2022
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.