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Title: Ric-8 controls Drosophila neural progenitor asymmetric division by regulating heterotrimeric G proteins
Authors: Wang, H. 
Ng, K.H.
Qian, H.
Siderovski, D.P.
Chia, W. 
Yu, F. 
Issue Date: Nov-2005
Citation: Wang, H., Ng, K.H., Qian, H., Siderovski, D.P., Chia, W., Yu, F. (2005-11). Ric-8 controls Drosophila neural progenitor asymmetric division by regulating heterotrimeric G proteins. Nature Cell Biology 7 (11) : 1091-1098. ScholarBank@NUS Repository.
Abstract: Asymmetric division of Drosophila neuroblasts (NBs) and the Caenorhabditis elegans zygote uses polarity cues provided by the Par proteins, as well as heterotrimeric G-protein-signalling that is activated by a receptor-independent mechanism mediated by GoLoco/GPR motif proteins. Another key component of this non-canonical G-protein activation mechanism is a non-receptor guanine nucleotide-exchange factor (GEF) for Gα, RIC-8, which has recently been characterized in C. elegans and in mammals3-6. We show here that the Drosophila Ric-8 homologue is required for asymmetric division of both NBs and pI cells. Ric-8 is necessary for membrane targeting of Gαi, Pins and Gβ13F, presumably by regulating multiple Gα subunit(s). Ric-8 forms an in vivo complex with Gαi and interacts preferentially with GDP-Gαi, which is consistent with Ric-8 acting as a GEF for Gαi. Comparisons of the phenotypes of Gαi, Ric-8, Gβ13F single and Ric-8;Gβ13F double loss-of-function mutants indicate that, in NBs, Ric-8 positively regulates Gαi activity. In addition, Gβ acts to restrict Gαi (and GoLoco proteins) to the apical cortex, where Gαi (and Pins) can mediate asymmetric spindle geometry. © 2005 Nature Publishing Group.
Source Title: Nature Cell Biology
ISSN: 14657392
DOI: 10.1038/ncb1317
Appears in Collections:Staff Publications

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