Please use this identifier to cite or link to this item: https://doi.org/10.1006/bbrc.2001.5309
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dc.titlePivotal role of mitochondrial Ca2+ in microcystin-induced mitochondrial permeability transition in rat hepatocytes
dc.contributor.authorDing, W.-X.
dc.contributor.authorShen, H.-M.
dc.contributor.authorOng, C.-N.
dc.date.accessioned2014-12-01T06:56:09Z
dc.date.available2014-12-01T06:56:09Z
dc.date.issued2001
dc.identifier.citationDing, W.-X., Shen, H.-M., Ong, C.-N. (2001). Pivotal role of mitochondrial Ca2+ in microcystin-induced mitochondrial permeability transition in rat hepatocytes. Biochemical and Biophysical Research Communications 285 (5) : 1155-1161. ScholarBank@NUS Repository. https://doi.org/10.1006/bbrc.2001.5309
dc.identifier.issn0006291X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/113586
dc.description.abstractWe have shown earlier that microcystin-LR (MLR), a specific hepatotoxin, induced onset of mitochondrial permeability transition (MPT) and apoptosis in rat hepatocytes. Here we attempted to investigate the role of mitochondrial Ca2+ in MLR-induced onset of MPT and cell death. Using confocal microscopy, we found that MLR caused an early surge of mitochondrial Ca2+ prior to the onset of MPT and cell death. Pretreatment with 1,2-bis(O-aminophenoxyl)ethane-N,N,N′,N′-tetracetic acid tetra(acetoxymethyl)ester (an intracellular Ca2+ chelator) or ruthenium red (an inhibitor of mitochondrial Ca2x uniporter) prevented the early mitochondrial Ca2+ surge and attenuated the subsequent onset of MPT and cell death. On the other hand, a mitochondrial uncoupler, CCCP, rapidly disrupted the mitochondrial membrane potential and also prevented the mitoehondrial Ca2+ surge, onset of MPT, and cell death. We thus conclude that mitochondrial Ca2+ plays an important role in the onset of MPT and cell death in MLR-treated rat hepatocytes. © 2001 Academic Press.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1006/bbrc.2001.5309
dc.sourceScopus
dc.subjectApoptosis
dc.subjectConfocal microscopy
dc.subjectCyanobacteria
dc.subjectMitochondrial membrane potential
dc.subjectOxidative stress
dc.typeArticle
dc.contributor.departmentCOMMUNITY,OCCUPATIONAL & FAMILY MEDICINE
dc.description.doi10.1006/bbrc.2001.5309
dc.description.sourcetitleBiochemical and Biophysical Research Communications
dc.description.volume285
dc.description.issue5
dc.description.page1155-1161
dc.description.codenBBRCA
dc.identifier.isiut000170300900013
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