Please use this identifier to cite or link to this item: https://doi.org/10.1006/enrs.1999.4025
Title: Involvement of oxidative stress in crystalline silica-induced cytotoxicity and genotoxicity in rat alveolar macrophages
Authors: Zhang, Z.
Shen, H.-M. 
Zhang, Q.-F.
Ong, C.-N. 
Keywords: Alveolar macrophage
Crystalline silica
Cytotoxicity
Genotoxicity
Oxidative stress
Reactive oxygen species
Issue Date: Mar-2000
Citation: Zhang, Z., Shen, H.-M., Zhang, Q.-F., Ong, C.-N. (2000-03). Involvement of oxidative stress in crystalline silica-induced cytotoxicity and genotoxicity in rat alveolar macrophages. Environmental Research 82 (3) : 245-252. ScholarBank@NUS Repository. https://doi.org/10.1006/enrs.1999.4025
Abstract: Alveolar macrophages (AMs) occupy a key position in silica-induced pulmonary fibrosis, although the mechanism3 are yet to be elucidated. In the present study we examined the involvement of oxidative stress and reactive oxygen species formation in silica-induced cytotoxicity and genotoxicity in cultured rat AMs. A lucigenin-dependent chemiluminescence test was used to determine superoxide anion (O2/-), and a 2',7'-dichlorofluorescin diacetate fluorescence test was employed to measure the hydrogen peroxide (H2O2) level. The cytotoxic and genotoxic effects caused by silica in AMs were examined by lactate dehydrogenase (LDH) leakage and single-cell gel electrophoresis (comet assay), respectively. The results showed that silica enhanced O2/- and H2O2 formation in AMs. There were clear dose- and time- dependent relationships in silica-induced cytotoxicity and genotoxicity. Furthermore, superoxide dismutase and catalase were able to reduce silica- induced LDH leakage and DNA damage, with concurrent significant inhibition on silica-induced oxidative stress in AMs. These findings provide convincing evidence that oxidative stress mediates the silica-induced cytotoxicity and genotoxicity. The understanding of such a mechanism may provide a scientific basis for the possible application of antioxidants in preventing the hazardous effects of silica. (C) 2000 Academic Press.
Source Title: Environmental Research
URI: http://scholarbank.nus.edu.sg/handle/10635/113535
ISSN: 00139351
DOI: 10.1006/enrs.1999.4025
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

42
checked on Dec 5, 2022

WEB OF SCIENCETM
Citations

36
checked on Dec 5, 2022

Page view(s)

118
checked on Dec 8, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.