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|Title:||Inhibition of aflatoxin B1-induced cell injury by selenium: An in vitro study||Authors:||Shi, C.-Y.
|Issue Date:||1995||Citation:||Shi, C.-Y.,Hew, Y.-C.,Ong, C.-N. (1995). Inhibition of aflatoxin B1-induced cell injury by selenium: An in vitro study. Human and Experimental Toxicology 14 (1) : 55-60. ScholarBank@NUS Repository.||Abstract:||Dietary selenium is an essential trace element in human nutrition. Selenium has been shown in animal studies to inhibit aflatoxin hepatocarcinogenesis. However, the cellular mechanism responsible for the inhibition has not been thoroughly studied. This study examines the effect of two selenium compounds, namely, sodium selenite and selenium-enriched yeast extract (SeY), on the cytotoxicity, DNA-binding and mutagenicity of aflatoxin B1 (AFB1) in cultured Chinese hamster ovary (CHO) cells. CHO cells, after treatment with 2 μg ml-1 selenite or 80 μg ml-1 SeY, exhibited increased resistance to AFB1-induced cell killing. At a concentration of 50 μg ml-1 AFB1, cell survival, measured by the clonogenicity assay, was increased by 21- and 10-fold in selenite- and SeY-treated cells, respectively. However, selenium treatment did not appear to affect AFB1-DNA binding. Similarly, no effect was observed on AFB1 mutagenicity, as determined by the hypoxanthineguanine phosphoribosyl transferase (HPRT) gene mutation assay. The results showed that selenium could effectively protect cells from AFB1 cytotoxicity in cultured cells but had no effect on AFB1 DNA adduct formation or mutagenesis. It is suggested that there are multiple pathways of AFB1 toxicity and that selenium can modulate AFB1-induced cell killing independent of its genotoxicity.||Source Title:||Human and Experimental Toxicology||URI:||http://scholarbank.nus.edu.sg/handle/10635/113525||ISSN:||01445952|
|Appears in Collections:||Staff Publications|
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