Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/113500
Title: High-performance liquid Chromatographie method for determination of dehydroabietic and abietic acids, the skin sensitizers in bindi adhesive
Authors: Lee, B.L.
Ong, H.Y.
Koh, D. 
Ong, C.N. 
Issue Date: 18-Nov-1994
Citation: Lee, B.L.,Ong, H.Y.,Koh, D.,Ong, C.N. (1994-11-18). High-performance liquid Chromatographie method for determination of dehydroabietic and abietic acids, the skin sensitizers in bindi adhesive. Journal of Chromatography A 685 (2) : 263-269. ScholarBank@NUS Repository.
Abstract: A sensitive and selective high-performance liquid Chromatographic (HPLC) method was developed for simultaneous determination of two common allergens, dehydroabietic (DHAA) and abietic acid in bindi adhesive with fluorimetric and ultraviolet (UV) absorbance detection. Bindi is a cosmetic item used by Indian women on their foreheads. The sample was ultrasonicated with 1 ml of acetonitrile for 30 min. A 0.6-ml aliquot of the extract was mixed with an equal volume of deionized water. After centrifugation, 1 ml of the supernatant was percolated through a preconditioned C18 solid-phase extraction column. After rinsing the column with 1 ml of acetonitrile-water (60:40. v v), the analytes were eluted with 1 ml of mobile phase A and 50 μl of the eluent was used for HPLC analysis. The two mobile phases used for gradient elution were: (A) methanoi-water (90:10, v v) containing 0.06% ( v v) formic acid and (B) methanol-water (75:25, v v) with 0.1% ( v v) formic acid. The flow-rate was set at 1.0 ml min. DHAA was detected with fluorescence (excitation 225 nm and emission 285 nm) at 3.6 min. Abietic acid was detected at 6.1 min with UV at 238 nm. The lowest detection limits (signal-to-noise ratio 3) were 0.5 and 1.25 ng for DHAA and abietic acid, respectively. Analytical recovery and reproducibility generally exceeded 95 and 90%, respectively. DHAA and abietic acid were present in most of the bindi samples tested, with mean values 0.36 μg of DHAA (n = 26) and 0.31 (μg of abietic acid (n = 24) per sample. © 1994.
Source Title: Journal of Chromatography A
URI: http://scholarbank.nus.edu.sg/handle/10635/113500
ISSN: 00219673
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

63
checked on Mar 28, 2020

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.