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Title: HEP-COP, a novel human gene whose product is highly homologous to the α-subunit of the yeast coatomer protein complex
Authors: Chow, V.T.K.
Quek, H.H. 
Chow, V.T.K.
Keywords: cDNA sequence
Eukaryotic secretory pathway
Hepatocellular carcinoma
Intracellular membrane transport
Rapid amplification of cDNA ends
WD-40 repeats
Issue Date: 9-Mar-1996
Citation: Chow, V.T.K., Quek, H.H., Chow, V.T.K. (1996-03-09). HEP-COP, a novel human gene whose product is highly homologous to the α-subunit of the yeast coatomer protein complex. Gene 169 (2) : 223-227. ScholarBank@NUS Repository.
Abstract: A 4333-bp novel human cDNA sequence designated HEP-COP was isolated from the Hep3B hepatocellular carcinoma cell line by the RACE technique. Within HEP-COP was identified an ORF of 3672 bp encoding a deduced 1224-amino-acid (aa) sequence which exhibited striking homology with the 1201-aa sequence of RET1P, the α-subunit of the coatomer complex (α-COP) in Saccharomyces cerevisiae which participates in membrane transport between the endoplasmic reticulum and Golgi apparatus. The aa homology was highest in their N-terminal regions which each contained six WD-40 repeat motifs [Van der Voorn and Ploegh, FEBS Lett. 307 (1992) 131-134], and both proteins were predicted to be hydrophilic with similar estimated molecular masses of 138 324 and 135 599 Da, respectively. Northern blot hybridization demonstrated that HEP-COP was expressed in a wide range of human adult and fetal tissues. RT-PCR analysis revealed no differential expression of HEP-COP in 14 human cancer cell lines, as compared with normal control cells. Considering the close similarities between HEP-COP and yeast α-COP, and the ubiquitous expression of HEP-COP implying an essential cellular role, it is likely that HEP-COP is the human homologue of α-COP.
Source Title: Gene
ISSN: 03781119
DOI: 10.1016/0378-1119(95)00738-5
Appears in Collections:Staff Publications

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