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https://doi.org/10.1016/0378-1119(95)00738-5
Title: | HEP-COP, a novel human gene whose product is highly homologous to the α-subunit of the yeast coatomer protein complex | Authors: | Chow, V.T.K. Quek, H.H. Chow, V.T.K. |
Keywords: | cDNA sequence Eukaryotic secretory pathway Hepatocellular carcinoma Intracellular membrane transport Rapid amplification of cDNA ends WD-40 repeats |
Issue Date: | 9-Mar-1996 | Citation: | Chow, V.T.K., Quek, H.H., Chow, V.T.K. (1996-03-09). HEP-COP, a novel human gene whose product is highly homologous to the α-subunit of the yeast coatomer protein complex. Gene 169 (2) : 223-227. ScholarBank@NUS Repository. https://doi.org/10.1016/0378-1119(95)00738-5 | Abstract: | A 4333-bp novel human cDNA sequence designated HEP-COP was isolated from the Hep3B hepatocellular carcinoma cell line by the RACE technique. Within HEP-COP was identified an ORF of 3672 bp encoding a deduced 1224-amino-acid (aa) sequence which exhibited striking homology with the 1201-aa sequence of RET1P, the α-subunit of the coatomer complex (α-COP) in Saccharomyces cerevisiae which participates in membrane transport between the endoplasmic reticulum and Golgi apparatus. The aa homology was highest in their N-terminal regions which each contained six WD-40 repeat motifs [Van der Voorn and Ploegh, FEBS Lett. 307 (1992) 131-134], and both proteins were predicted to be hydrophilic with similar estimated molecular masses of 138 324 and 135 599 Da, respectively. Northern blot hybridization demonstrated that HEP-COP was expressed in a wide range of human adult and fetal tissues. RT-PCR analysis revealed no differential expression of HEP-COP in 14 human cancer cell lines, as compared with normal control cells. Considering the close similarities between HEP-COP and yeast α-COP, and the ubiquitous expression of HEP-COP implying an essential cellular role, it is likely that HEP-COP is the human homologue of α-COP. | Source Title: | Gene | URI: | http://scholarbank.nus.edu.sg/handle/10635/113499 | ISSN: | 03781119 | DOI: | 10.1016/0378-1119(95)00738-5 |
Appears in Collections: | Staff Publications |
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