Please use this identifier to cite or link to this item:
https://doi.org/10.1016/S1383-5718(03)00055-X
DC Field | Value | |
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dc.title | Exposure to acrylonitrile induced DNA strand breakage and sex chromosome aneuploidy in human spermatozoa | |
dc.contributor.author | Xu, D.-X. | |
dc.contributor.author | Zhu, Q.-X. | |
dc.contributor.author | Zheng, L.-K. | |
dc.contributor.author | Wang, Q.-N. | |
dc.contributor.author | Shen, H.-M. | |
dc.contributor.author | Deng, L.-X. | |
dc.contributor.author | Ong, C.-N. | |
dc.date.accessioned | 2014-12-01T06:54:53Z | |
dc.date.available | 2014-12-01T06:54:53Z | |
dc.date.issued | 2003-05-09 | |
dc.identifier.citation | Xu, D.-X., Zhu, Q.-X., Zheng, L.-K., Wang, Q.-N., Shen, H.-M., Deng, L.-X., Ong, C.-N. (2003-05-09). Exposure to acrylonitrile induced DNA strand breakage and sex chromosome aneuploidy in human spermatozoa. Mutation Research - Genetic Toxicology and Environmental Mutagenesis 537 (1) : 93-100. ScholarBank@NUS Repository. https://doi.org/10.1016/S1383-5718(03)00055-X | |
dc.identifier.issn | 13835718 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/113472 | |
dc.description.abstract | To explore acrylonitrile (ACN)-induced DNA strand breakage and sex chromosome aneuploidy in human spermatozoa, semen parameters were examined among 30 acrylonitrile-exposed workers according to WHO laboratory manual for the examination of human sperm. DNA strand breakage of sperm cells was investigated among 30 ACN-exposed workers using single cell gel electrophoresis (SCGE). The frequency of sex chromosome aneuploidy in sperm cells was analyzed among nine ACN-exposed workers using fluorescence in situ hybridization (FISH). The geometrical mean of sperm density was 75×106ml-1 in exposure group, significantly lower than 140×106ml-1 in the control. The geometrical mean of sperm number per ejaculum was 205×106 in exposure group, significantly lower than 280×106 in the control. The rates of comet sperm nuclei were 28.7% in exposure group, significantly higher than 15.0% in the control. Mean tail length was 9.8μm in exposure group, longer than 4.3μm in the control. The frequency of sex chromosome disomy was 0.69% in exposure group, significantly higher than 0.35% in the control. XY-bearing sperm was the most common sex chromosome disomy, with an average rate of 0.37% in exposure group, and 0.20% in the control. XX- and YY-bearing sperm accounted for an additional 0.09 and 0.23% in exposure group, and 0.05 and 0.10% in the control. The results indicate that ACN affect semen quality among ACN-exposed workers. ACN or its metabolites could induce reproductive defects as an in vivo multipotent genotoxic agent by inducing DNA strand breakage and sex chromosome non-disjunction in spermatogenesis. © 2003 Elsevier Science B.V. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S1383-5718(03)00055-X | |
dc.source | Scopus | |
dc.subject | Acrylonitrile | |
dc.subject | Aneuploidy | |
dc.subject | Chromosome | |
dc.subject | DNA damage | |
dc.subject | Sperm | |
dc.type | Article | |
dc.contributor.department | COMMUNITY,OCCUPATIONAL & FAMILY MEDICINE | |
dc.description.doi | 10.1016/S1383-5718(03)00055-X | |
dc.description.sourcetitle | Mutation Research - Genetic Toxicology and Environmental Mutagenesis | |
dc.description.volume | 537 | |
dc.description.issue | 1 | |
dc.description.page | 93-100 | |
dc.description.coden | MRGMF | |
dc.identifier.isiut | 000183254000009 | |
Appears in Collections: | Staff Publications |
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