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|Title:||Exposure to acrylonitrile induced DNA strand breakage and sex chromosome aneuploidy in human spermatozoa||Authors:||Xu, D.-X.
|Issue Date:||9-May-2003||Citation:||Xu, D.-X., Zhu, Q.-X., Zheng, L.-K., Wang, Q.-N., Shen, H.-M., Deng, L.-X., Ong, C.-N. (2003-05-09). Exposure to acrylonitrile induced DNA strand breakage and sex chromosome aneuploidy in human spermatozoa. Mutation Research - Genetic Toxicology and Environmental Mutagenesis 537 (1) : 93-100. ScholarBank@NUS Repository. https://doi.org/10.1016/S1383-5718(03)00055-X||Abstract:||To explore acrylonitrile (ACN)-induced DNA strand breakage and sex chromosome aneuploidy in human spermatozoa, semen parameters were examined among 30 acrylonitrile-exposed workers according to WHO laboratory manual for the examination of human sperm. DNA strand breakage of sperm cells was investigated among 30 ACN-exposed workers using single cell gel electrophoresis (SCGE). The frequency of sex chromosome aneuploidy in sperm cells was analyzed among nine ACN-exposed workers using fluorescence in situ hybridization (FISH). The geometrical mean of sperm density was 75×106ml-1 in exposure group, significantly lower than 140×106ml-1 in the control. The geometrical mean of sperm number per ejaculum was 205×106 in exposure group, significantly lower than 280×106 in the control. The rates of comet sperm nuclei were 28.7% in exposure group, significantly higher than 15.0% in the control. Mean tail length was 9.8μm in exposure group, longer than 4.3μm in the control. The frequency of sex chromosome disomy was 0.69% in exposure group, significantly higher than 0.35% in the control. XY-bearing sperm was the most common sex chromosome disomy, with an average rate of 0.37% in exposure group, and 0.20% in the control. XX- and YY-bearing sperm accounted for an additional 0.09 and 0.23% in exposure group, and 0.05 and 0.10% in the control. The results indicate that ACN affect semen quality among ACN-exposed workers. ACN or its metabolites could induce reproductive defects as an in vivo multipotent genotoxic agent by inducing DNA strand breakage and sex chromosome non-disjunction in spermatogenesis. © 2003 Elsevier Science B.V. All rights reserved.||Source Title:||Mutation Research - Genetic Toxicology and Environmental Mutagenesis||URI:||http://scholarbank.nus.edu.sg/handle/10635/113472||ISSN:||13835718||DOI:||10.1016/S1383-5718(03)00055-X|
|Appears in Collections:||Staff Publications|
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