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Title: Effects of storage time on stability of salivary immunoglobulin A and lysozyme
Authors: Ng, V.
Koh, D. 
Fu, Q.
Chia, S.-E. 
Keywords: Salivary biomarkers
Salivary IgA
Salivary lysozyme
Storage duration
Issue Date: Dec-2003
Citation: Ng, V., Koh, D., Fu, Q., Chia, S.-E. (2003-12). Effects of storage time on stability of salivary immunoglobulin A and lysozyme. Clinica Chimica Acta 338 (1-2) : 131-134. ScholarBank@NUS Repository.
Abstract: Background: In many research settings, storage of samples prior to analysis is unavoidable. This study investigates the effects of storage time on stability of salivary immunoglobulin A (IgA) and lysozyme. Methods: Saliva samples were obtained from 30 healthy adults. Each sample was divided into five aliquots and stored at -30°C until analysis. The samples were measured for IgA and lysozyme concentrations after 1, 2, 3, 8 and 12 months of storage using enzyme-linked immunosorbent assay. Results: There was a decline in the concentrations of IgA and lysozyme with increasing storage time. Repeated measures analyses for both salivary IgA and lysozyme showed a significant difference after 8 months of storage as compared to the 1st month (p10% after storage for 8 months or more. A similar pattern was observed for lysozyme with % change in majority of the samples >14% levels when the samples were assayed at 8th month and beyond (mean% change±S.D.>14%). Conclusion: Salivary IgA and lysozyme concentrations remain stable for up to 3 months when stored at -30°C. These findings have important implications with regard to measurement validity of salivary biomarkers research. © 2003 Elsevier B.V. All rights reserved.
Source Title: Clinica Chimica Acta
ISSN: 00098981
DOI: 10.1016/j.cccn.2003.08.012
Appears in Collections:Staff Publications

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