Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.neulet.2012.08.006
Title: Detectability of secretagogin in human erythrocytes
Authors: Tan, W.S.D.
Lee, J.J.
Satish, R.L. 
Ang, E.-T.
Keywords: Ageing
Calcium-binding protein
EF-hand
Erythrocyte
Secretagogin
Issue Date: 20-Sep-2012
Citation: Tan, W.S.D., Lee, J.J., Satish, R.L., Ang, E.-T. (2012-09-20). Detectability of secretagogin in human erythrocytes. Neuroscience Letters 526 (1) : 59-62. ScholarBank@NUS Repository. https://doi.org/10.1016/j.neulet.2012.08.006
Abstract: Secretagogin is a six EF-hand calcium-binding protein that can identify granule cells in the dentate gyrus of hippocampus. The aim of this study was to determine if secretagogin can be detected in human blood cells. Eight adult males were recruited for blood analysis. Whole blood was separated into plasma, peripheral mononuclear cells and erythrocytes with Ficoll-Paque and probed for secretagogin using reverse-transcription polymerase chain reaction and Western blot. While secretagogin mRNA was detected in both peripheral mononuclear cells and erythrocytes using reverse-transcription polymerase chain reaction, SCGN protein was only detected in erythrocytes. Interestingly, peripheral mononuclear cells secretagogin mRNA expression levels showed significant negative correlation with age. This begets the question on the function of secretagogin in blood cells and if it is correlated to neurodegeneration associated with ageing. This remains our impetus for further research. © 2012 Elsevier Ireland Ltd.
Source Title: Neuroscience Letters
URI: http://scholarbank.nus.edu.sg/handle/10635/113428
ISSN: 03043940
DOI: 10.1016/j.neulet.2012.08.006
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