Please use this identifier to cite or link to this item: https://doi.org/10.1073/pnas.0408229102
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dc.titleCaspases and nitric oxide broadly regulate dendritic cell maturation and surface expression of class II MHC proteins
dc.contributor.authorSiew, H.W.
dc.contributor.authorSantambrogio, L.
dc.contributor.authorStrominger, J.L.
dc.date.accessioned2014-12-01T06:53:57Z
dc.date.available2014-12-01T06:53:57Z
dc.date.issued2004-12-21
dc.identifier.citationSiew, H.W., Santambrogio, L., Strominger, J.L. (2004-12-21). Caspases and nitric oxide broadly regulate dendritic cell maturation and surface expression of class II MHC proteins. Proceedings of the National Academy of Sciences of the United States of America 101 (51) : 17783-17788. ScholarBank@NUS Repository. https://doi.org/10.1073/pnas.0408229102
dc.identifier.issn00278424
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/113391
dc.description.abstractThe passage of dendritic cells (DC) from immature to terminally differentiated antigen-presenting cells is accompanied by numerous morphological, phenotypic, and functional changes. These changes include, for example, expression of "empty" class II MHC proteins (MHCII) at the surface in immature DC, whereas a much larger amount of peptide-loaded MHCII is expressed at the surface in mature DC. Here we show that, in cultured immature DC derived from murine bone-marrow precursors, a number of molecules involved in intracellular trafficking were present in a cleaved form, degraded by caspase-like proteases. Cleavage was either inhibited or reduced significantly during maturation of DC induced by either LPS and TNF-α or by peptides that inhibit caspase activities. Inducible nitric oxide (NO) synthetase up-regulated by LPS was essential for inhibiting the caspase-like activity during the maturation of DC. Moreover, treatment with LPS or caspase inhibitor resulted in expression of MHCII/peptide complexes at the cell surface. Thus, the alteration of the endosomal trafficking pathways during the development of DC that parallels the changes in surface expression of MHCII is regulated at least in part by the activities of caspases, inducible NO synthetase, and its product NO.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1073/pnas.0408229102
dc.sourceScopus
dc.subjectAntigen presentation
dc.subjectEndosomes
dc.subjectLPS
dc.subjectNitric oxide synthetase
dc.subjectProtease inhibitors
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1073/pnas.0408229102
dc.description.sourcetitleProceedings of the National Academy of Sciences of the United States of America
dc.description.volume101
dc.description.issue51
dc.description.page17783-17788
dc.description.codenPNASA
dc.identifier.isiut000225951500040
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