Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/113355
Title: Amplification of cDNA via RT-PCR using RNA extracted from postmortem tissues
Authors: Phang, T.-W.
Shi, C.-Y.
Chia, J.-N.
Ong, C.-N. 
Keywords: cDNA
pathology and biology
postmortem tissues
RNA extraction
RT-PCR
Issue Date: 1994
Citation: Phang, T.-W.,Shi, C.-Y.,Chia, J.-N.,Ong, C.-N. (1994). Amplification of cDNA via RT-PCR using RNA extracted from postmortem tissues. Journal of Forensic Sciences 39 (5) : 1275-1279. ScholarBank@NUS Repository.
Abstract: Analysis of cDNA derived from messenger RNA is of advantage over using genomic DNA in genetic analysis of large genes, especially those with lengthy intron sequences. However, because of its instability and rapid degradation, RNA extraction from postmortem tissues has not been attempted. Here, we report the successful extraction of intact mRNA from various postmortem tissues from accidental and sudden death cases. Subsequently with reverse transcriptase-polymerase chain reaction (RT-PCR), we were able to amplify cDNA fragments of different lengths up to 0.9 kb. The described method therefore provides a useful tool in genetic analysis of postmortem tissues.
Source Title: Journal of Forensic Sciences
URI: http://scholarbank.nus.edu.sg/handle/10635/113355
ISSN: 00221198
Appears in Collections:Staff Publications

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