Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/113215
DC Field | Value | |
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dc.title | AFP+, ESC-derived cells engraft and differentiate into hepatocytes in vivo | |
dc.contributor.author | Yin, Y. | |
dc.contributor.author | Yew, K.L. | |
dc.contributor.author | Salto-Tellez, M. | |
dc.contributor.author | Ng, S.C. | |
dc.contributor.author | Lin, C.-S. | |
dc.contributor.author | Lim, S.-K. | |
dc.date.accessioned | 2014-11-30T06:41:03Z | |
dc.date.available | 2014-11-30T06:41:03Z | |
dc.date.issued | 2002 | |
dc.identifier.citation | Yin, Y.,Yew, K.L.,Salto-Tellez, M.,Ng, S.C.,Lin, C.-S.,Lim, S.-K. (2002). AFP+, ESC-derived cells engraft and differentiate into hepatocytes in vivo. Stem Cells 20 (4) : 338-346. ScholarBank@NUS Repository. | |
dc.identifier.issn | 10665099 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/113215 | |
dc.description.abstract | A major problem in gene therapy and tissue replacement is accessibility of tissue-specific stem cells. One solution is to isolate tissue-specific stem cells from differentiating embryonic stem (ES) cells. Here, we show that liver progenitor cells can be purified from differentiated ES cells using alpha-fetoprotein (AFP) as a marker. By knocking the green fluorescent protein (GFP) gene into the AFP locus of ES cells and differentiating the modified ES cells in vitro, a subpopulation of GFP+ and AFP-expressing cells was generated. When transplanted into partially hepatectomized lacZ-positive ROSA26 mice, GFP+ cells engrafted and differentiated into lacZ-negative and albumin-positive hepatocytes. Differentiation into hepatocytes also occurred after transplantation of GFP+ cells in apolipoprotein-E-(ApoE) or haptoglobin-deficient mice as demonstrated by the presence of ApoE-positive hepatocytes and ApoE mRNA in the liver of ApoE-deficient mice or by haptoglobin in the serum and haptoglobin mRNA in the liver of haptoglobin-deficient mice. This study describes the first isolation of ES-cell-derived liver progenitor cells that are viable mediators of liver-specific functions in vivo. | |
dc.source | Scopus | |
dc.subject | Alpha-fetoprotein | |
dc.subject | Embryoid bodies | |
dc.subject | Embryonic stem cells | |
dc.subject | Green fluorescent protein | |
dc.subject | Hepatocytes | |
dc.type | Article | |
dc.contributor.department | GENOME INSTITUTE OF SINGAPORE | |
dc.description.sourcetitle | Stem Cells | |
dc.description.volume | 20 | |
dc.description.issue | 4 | |
dc.description.page | 338-346 | |
dc.description.coden | STCEE | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Staff Publications |
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