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https://doi.org/10.1371/journal.pone.0049885
Title: | Physical Organization of DNA by Multiple Non-Specific DNA-Binding Modes of Integration Host Factor (IHF) | Authors: | Lin, J. Chen, H. Dröge, P. Yan, J. |
Issue Date: | 14-Nov-2012 | Citation: | Lin, J., Chen, H., Dröge, P., Yan, J. (2012-11-14). Physical Organization of DNA by Multiple Non-Specific DNA-Binding Modes of Integration Host Factor (IHF). PLoS ONE 7 (11) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0049885 | Abstract: | The integration host factor (IHF) is an abundant nucleoid-associated protein and an essential co-factor for phage λ site-specific recombination and gene regulation in E. coli. Introduction of a sharp DNA kink at specific cognate sites is critical for these functions. Interestingly, the intracellular concentration of IHF is much higher than the concentration needed for site-specific interactions, suggesting that non-specific binding of IHF to DNA plays a role in the physical organization of bacterial chromatin. However, it is unclear how non-specific DNA association contributes to DNA organization. By using a combination of single DNA manipulation and atomic force microscopy imaging methods, we show here that distinct modes of non-specific DNA binding of IHF result in complex global DNA conformations. Changes in KCl and IHF concentrations, as well as tension applied to DNA, dramatically influence the degree of DNA-bending. In addition, IHF can crosslink DNA into a highly compact DNA meshwork that is observed in the presence of magnesium at low concentration of monovalent ions and high IHF-DNA stoichiometries. Our findings provide important insights into how IHF contributes to bacterial chromatin organization, gene regulation, and biofilm formation. © 2012 Lin et al. | Source Title: | PLoS ONE | URI: | http://scholarbank.nus.edu.sg/handle/10635/113130 | ISSN: | 19326203 | DOI: | 10.1371/journal.pone.0049885 |
Appears in Collections: | Staff Publications Elements |
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