Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0049885
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dc.titlePhysical Organization of DNA by Multiple Non-Specific DNA-Binding Modes of Integration Host Factor (IHF)
dc.contributor.authorLin, J.
dc.contributor.authorChen, H.
dc.contributor.authorDröge, P.
dc.contributor.authorYan, J.
dc.date.accessioned2014-11-28T09:12:23Z
dc.date.available2014-11-28T09:12:23Z
dc.date.issued2012-11-14
dc.identifier.citationLin, J., Chen, H., Dröge, P., Yan, J. (2012-11-14). Physical Organization of DNA by Multiple Non-Specific DNA-Binding Modes of Integration Host Factor (IHF). PLoS ONE 7 (11) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0049885
dc.identifier.issn19326203
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/113130
dc.description.abstractThe integration host factor (IHF) is an abundant nucleoid-associated protein and an essential co-factor for phage λ site-specific recombination and gene regulation in E. coli. Introduction of a sharp DNA kink at specific cognate sites is critical for these functions. Interestingly, the intracellular concentration of IHF is much higher than the concentration needed for site-specific interactions, suggesting that non-specific binding of IHF to DNA plays a role in the physical organization of bacterial chromatin. However, it is unclear how non-specific DNA association contributes to DNA organization. By using a combination of single DNA manipulation and atomic force microscopy imaging methods, we show here that distinct modes of non-specific DNA binding of IHF result in complex global DNA conformations. Changes in KCl and IHF concentrations, as well as tension applied to DNA, dramatically influence the degree of DNA-bending. In addition, IHF can crosslink DNA into a highly compact DNA meshwork that is observed in the presence of magnesium at low concentration of monovalent ions and high IHF-DNA stoichiometries. Our findings provide important insights into how IHF contributes to bacterial chromatin organization, gene regulation, and biofilm formation. © 2012 Lin et al.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1371/journal.pone.0049885
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentMECHANOBIOLOGY INSTITUTE
dc.contributor.departmentPHYSICS
dc.description.doi10.1371/journal.pone.0049885
dc.description.sourcetitlePLoS ONE
dc.description.volume7
dc.description.issue11
dc.description.page-
dc.identifier.isiut000311151900201
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