Please use this identifier to cite or link to this item:
Title: Dynamics and Regulation of RecA Polymerization and De-Polymerization on Double-Stranded DNA
Authors: Fu, H. 
Le, S.
Muniyappa, K.
Yan, J. 
Issue Date: 18-Jun-2013
Citation: Fu, H., Le, S., Muniyappa, K., Yan, J. (2013-06-18). Dynamics and Regulation of RecA Polymerization and De-Polymerization on Double-Stranded DNA. PLoS ONE 8 (6) : -. ScholarBank@NUS Repository.
Abstract: The RecA filament formed on double-stranded (ds) DNA is proposed to be a functional state analogous to that generated during the process of DNA strand exchange. RecA polymerization and de-polymerization on dsDNA is governed by multiple physiological factors. However, a comprehensive understanding of how these factors regulate the processes of polymerization and de-polymerization of RecA filament on dsDNA is still evolving. Here, we investigate the effects of temperature, pH, tensile force, and DNA ends (in particular ssDNA overhang) on the polymerization and de-polymerization dynamics of the E. coli RecA filament at a single-molecule level. Our results identified the optimal conditions that permitted spontaneous RecA nucleation and polymerization, as well as conditions that could maintain the stability of a preformed RecA filament. Further examination at a nano-meter spatial resolution, by stretching short DNA constructs, revealed a striking dynamic RecA polymerization and de-polymerization induced saw-tooth pattern in DNA extension fluctuation. In addition, we show that RecA does not polymerize on S-DNA, a recently identified novel base-paired elongated DNA structure that was previously proposed to be a possible binding substrate for RecA. Overall, our studies have helped to resolve several previous single-molecule studies that reported contradictory and inconsistent results on RecA nucleation, polymerization and stability. Furthermore, our findings also provide insights into the regulatory mechanisms of RecA filament formation and stability in vivo. © 2013 Fu et al.
Source Title: PLoS ONE
ISSN: 19326203
DOI: 10.1371/journal.pone.0066712
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
2013_Dynamics_and_Regulation_of_RecA-published.PDF1.2 MBAdobe PDF




checked on May 17, 2022


checked on May 12, 2022

Page view(s)

checked on May 12, 2022


checked on May 12, 2022

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.