Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/112949
Title: Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana
Authors: Brigneti, G.
Voinnet, O.
Li, W.-X.
Ji, L.-H. 
Ding, S.-W. 
Baulcombe, D.C.
Keywords: Cucumber mosaic virus
Gene silencing
Potato virus X
Potato virus Y
Virus resistance
Issue Date: 16-Nov-1998
Citation: Brigneti, G.,Voinnet, O.,Li, W.-X.,Ji, L.-H.,Ding, S.-W.,Baulcombe, D.C. (1998-11-16). Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana. EMBO Journal 17 (22) : 6739-6746. ScholarBank@NUS Repository.
Abstract: Post-transcriptional gene silencing (PTGS) of a green fluorescent protein (GFP) transgene is suppressed in Nicotiana benthamiana plants infected with potato virus Y (PVY) or with cucumber mosaic virus (CMV), but not in plants infected with potato virus X (PVX). By expressing PVY and CMV-encoded proteins in a PVX vector we have shown that the viral suppressors of gene silencing are the HCPro of PVY and the 2b protein of CMV. The HCPro acts by blocking the maintenance of PTGS in tissues where silencing had already been set, whereas the 2b protein prevents initiation of gene silencing at the growing points of the plants. Combined with previous findings that viruses are both activators and targets of PTGS, these data provide compelling evidence that PTGS represents a natural mechanism for plant protection against viruses.
Source Title: EMBO Journal
URI: http://scholarbank.nus.edu.sg/handle/10635/112949
ISSN: 02614189
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

47
checked on Nov 8, 2019

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.