Please use this identifier to cite or link to this item: https://doi.org/10.1093/emboj/18.10.2683
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dc.titleStrong host resistance targeted against a viral suppressor of the plant gene silencing defence mechanism
dc.contributor.authorLi, H.-W.
dc.contributor.authorLucy, A.P.
dc.contributor.authorGuo, H.-S.
dc.contributor.authorLi, W.-X.
dc.contributor.authorJi, L.-H.
dc.contributor.authorWong, S.-M.
dc.contributor.authorDing, S.-W.
dc.date.accessioned2014-11-28T08:12:29Z
dc.date.available2014-11-28T08:12:29Z
dc.date.issued1999-05-17
dc.identifier.citationLi, H.-W., Lucy, A.P., Guo, H.-S., Li, W.-X., Ji, L.-H., Wong, S.-M., Ding, S.-W. (1999-05-17). Strong host resistance targeted against a viral suppressor of the plant gene silencing defence mechanism. EMBO Journal 18 (10) : 2683-2691. ScholarBank@NUS Repository. https://doi.org/10.1093/emboj/18.10.2683
dc.identifier.issn02614189
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/112940
dc.description.abstractThe 2b protein encoded by cucumber mosaic cucumovirus (Cmv2b) acts as an important virulence determinant by suppressing post-transcriptional gene silencing (PTGS), a natural plant defence mechanism against viruses. We report here that the tomato aspermy cucumovirus 2b protein (Tav2b), when expressed from the unrelated tobacco mosaic tobamovirus (TMV) RNA genome, activates strong host resistance responses to TMV in tobacco which are typical of the gene-for-gene disease resistance mechanism. Domain swapping between Cmv2b, which does not elicit these responses, and Tav2b, revealed functional domains in Tav2b critical for triggering virus resistance and hypersensitive cell death. Furthermore, substitution of two amino acids from Tav2b by those found at the same positions in Cmv2b, Lys21→Val and Arg28→Ser, abolished the ability to induce hypersensitive cell death and virus resistance. However, in Nicotiana benthamiana, a species related to tobacco, Tav2b functions as a virulence determinant and suppresses PTGS. Thus, a viral suppressor of the host gene silencing defence mechanism is the target of another independent host resistance mechanism. Our results provide new insights into the complex molecular strategies employed by viruses and their hosts for defence, counter-defence and counter counter-defence.
dc.sourceScopus
dc.subjectAvirulence gene
dc.subjectCucumber mosaic virus
dc.subjectDisease resistance
dc.subjectGene silencing
dc.subjectHypersensitive response
dc.typeArticle
dc.contributor.departmentINSTITUTE OF MOLECULAR AGROBIOLOGY
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1093/emboj/18.10.2683
dc.description.sourcetitleEMBO Journal
dc.description.volume18
dc.description.issue10
dc.description.page2683-2691
dc.description.codenEMJOD
dc.identifier.isiut000080593800002
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