Please use this identifier to cite or link to this item:
|Title:||Proteolytic mapping of the coronavirus infectious bronchitis virus lb polyprotein: Evidence for the presence of four cleavage sites of the 3C-like proteinase and identification of two novel cleavage products||Authors:||Liu, D.X.
|Issue Date:||5-Jul-1998||Citation:||Liu, D.X., Shen, S., Xu, H.Y., Wang, S.F. (1998-07-05). Proteolytic mapping of the coronavirus infectious bronchitis virus lb polyprotein: Evidence for the presence of four cleavage sites of the 3C-like proteinase and identification of two novel cleavage products. Virology 246 (2) : 288-297. ScholarBank@NUS Repository. https://doi.org/10.1006/viro.1998.9199||Abstract:||We have previously reported that the 3C-like proteinase of the coronavirus infectious bronchitis virus (IBV) is responsible for processing of the 1a and 1a/1b polyproteins to three mature products of 24, 10, and 100 kDa (Liu et al., 1994, 1997; Ng and Liu, 1998). The C-terminal cleavage site of the 100-kDa protein was defined to be the Q(891(1b))-S(892(1b)) dipeptide bond encoded by nucleotides 15,129 to 15,134 (Liu and Brown, 1995). In this report, other cleavage sites of the 3C-like proteinase in the polyprotein encoded by the ORF 1b region were mapped by coexpression, deletion, and site- directed mutagenesis studies. Using two ORF 1b-specific antisera, V58 and V17, three more Q-S(G) dipeptide bonds, encoded by nucleotides 16,929 to 16,934, 18,492 to 18,497, and 19,506 to 19,511, respectively, were demonstrated to be the cleavage sites of the 3C-like proteinase. Cleavage at these four positions would result in the release of four mature products with molecular masses of approximately 68, 58, 39, and 35 kDa. Among them, the 39- and 35-kDa proteins were specifically identified in IBV-infected cells. Taken together with the 100-kDa protein previously identified, these results suggest that the ORF 1b region of IBV mRNA1 may be able to encode five mature products.||Source Title:||Virology||URI:||http://scholarbank.nus.edu.sg/handle/10635/112937||ISSN:||00426822||DOI:||10.1006/viro.1998.9199|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Oct 18, 2021
WEB OF SCIENCETM
checked on Oct 18, 2021
checked on Oct 14, 2021
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.