Please use this identifier to cite or link to this item: https://doi.org/10.1104/pp.005538
Title: Molecular characterization of the cotton GhTUB1 gene that is preferentially expressed in fiber
Authors: Li, X.-B.
Cai, L.
Cheng, N.-H.
Liu, J.-W. 
Issue Date: Oct-2002
Citation: Li, X.-B., Cai, L., Cheng, N.-H., Liu, J.-W. (2002-10). Molecular characterization of the cotton GhTUB1 gene that is preferentially expressed in fiber. Plant Physiology 130 (2) : 666-674. ScholarBank@NUS Repository. https://doi.org/10.1104/pp.005538
Abstract: Each fiber of cotton (Gossypium hirsutum) is a single epidermal cell that rapidly elongates to 2.5 to 3.0 cm from the ovule surface within about 16 d after anthesis. A large number of genes are required for fiber differentiation and development, but so far, little is known about how these genes control and regulate the process of fiber development. To investigate gene expression patterns in fiber, a cDNA, GhTUB1, encoding β-tubulin was isolated from a cotton fiber cDNA library. The analyses of RNA northern-blot hybridization and reverse transcriptase-polymerase chain reaction demonstrated that GhTUB1 transcripts preferentially accumulated at high levels in fiber, at low levels in ovules at the early stage of cotton boll development, and at very low levels in other tissues of cotton. The corresponding GhTUB1 gene including the promoter region was isolated by screening a cotton genomic DNA library. To demonstrate the specificity of the GhTUB1 promoter, the 5′-flanking region including the promoter and 5′-untranslated region was fused with the β-glucuronidase reporter gene. The expression of the reporter chimera was examined in a large number of transgenic cotton plants. Histochemical assays demonstrated that GhTUB1
Source Title: Plant Physiology
URI: http://scholarbank.nus.edu.sg/handle/10635/112933
ISSN: 00320889
DOI: 10.1104/pp.005538
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