Please use this identifier to cite or link to this item: https://doi.org/10.1074/jbc.271.24.14183
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dc.titleUnusual amino acid determinants of host range in the Mtx2 family of mosquitocidal toxins
dc.contributor.authorChan, S.W.
dc.contributor.authorThanabalu, T.
dc.contributor.authorWee, B.Y.
dc.contributor.authorPorter, A.G.
dc.date.accessioned2014-11-28T02:53:47Z
dc.date.available2014-11-28T02:53:47Z
dc.date.issued1996
dc.identifier.citationChan, S.W., Thanabalu, T., Wee, B.Y., Porter, A.G. (1996). Unusual amino acid determinants of host range in the Mtx2 family of mosquitocidal toxins. Journal of Biological Chemistry 271 (24) : 14183-14187. ScholarBank@NUS Repository. https://doi.org/10.1074/jbc.271.24.14183
dc.identifier.issn00219258
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/112153
dc.description.abstractFive different mosquitocidal toxin (mtx2) gene homologs have been cloned from eight Bacillus sphaericus strains. Pairwise comparisons of the predicted amino acid sequences show between four and eight substitutions compared with the prototype Mtx2 from B. sphaericus strain SSII-1. Mtx2 from strain SSII-1 was ~7-fold more toxic to Culex mosquito larvae than the Mtx2 homolog from B. sphaericus strain 31-2. Conversely, Mtx2 from strain 31-2 was ~100-fold more toxic to Aedes mosquito larvae than Mtx2 from strain SSII-1. Lys224 in Mtx2 was found to be the most important amino acid for toxicity to Culex larvae, and substitution of Lys224 with threonine abolished the toxicity of Mtx2 from strain SSII-1 to these larvae. In complete contrast, Thr224 was found to be crucial for the toxicity of Mtx2 from strain 31-2 to Aedes larvae, and substitution of Thr224 with lysine caused a ~100-fold drop in toxicity to these larvae. Thus, amino acid 224 in the Mtx2 family of mosquitocidal toxins is an unusual and important determinant of mosquito larvicidal activity and host range.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1074/jbc.271.24.14183
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentINSTITUTE OF MOLECULAR & CELL BIOLOGY
dc.description.doi10.1074/jbc.271.24.14183
dc.description.sourcetitleJournal of Biological Chemistry
dc.description.volume271
dc.description.issue24
dc.description.page14183-14187
dc.description.codenJBCHA
dc.identifier.isiutA1996UQ66000039
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