Please use this identifier to cite or link to this item: https://doi.org/10.1007/BF00023395
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dc.titleThree genes encode 3-hydroxy-3-methylglutaryl-coenzyme A reductase in Hevea brasiliensis: hmg1 and hmg3 are differentially expressed
dc.contributor.authorChye, M.-L.
dc.contributor.authorTan, C.-T.
dc.contributor.authorChua, N.-H.
dc.date.accessioned2014-11-28T02:53:38Z
dc.date.available2014-11-28T02:53:38Z
dc.date.issued1992-06
dc.identifier.citationChye, M.-L., Tan, C.-T., Chua, N.-H. (1992-06). Three genes encode 3-hydroxy-3-methylglutaryl-coenzyme A reductase in Hevea brasiliensis: hmg1 and hmg3 are differentially expressed. Plant Molecular Biology 19 (3) : 473-484. ScholarBank@NUS Repository. https://doi.org/10.1007/BF00023395
dc.identifier.issn01674412
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/112139
dc.description.abstractThe enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) catalyses an important step in isoprenoid biosynthesis in plants. In Hevea brasiliensis, HMGR is encoded by a small gene family comprised of three members, hmg1, hmg2 and hmg3. We have previously described hmg1 and hmg2 (Plant Mol Biol 16: 567-577, 1991). Here we report the isolation and characterization of hmg3 genomic and cDNA clones. In comparison to hmg1 which is more highly expressed in laticifers than in leaves, the level of hmg3 mRNA level is equally abundant in laticifers and leaves. In situ hybridization experiments showed that the expression of hmg3 is not cell-type specific while hmg1 is expressed predominantly in the laticifers. Primer-extension experiments using laticifer RNA showed that hmg1 is induced by ethylene while hmg3 expression remains constitutive. The hmg3 promoter, like the promoters of most house-keeping genes, lacks a TATA box. Our results suggest that hmg1 is likely to encode the enzyme involved in rubber biosynthesis while hmg3 is possibly involved in isoprenoid biosynthesis of a housekeeping nature. © 1992 Kluwer Academic Publishers.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/BF00023395
dc.sourceScopus
dc.subjectcis-1,4-polyisoprene
dc.subjectethylene induction
dc.subjectisoprenoid biosynthesis
dc.subjectlatex
dc.subjectlaticifer
dc.subjectnatural rubber
dc.typeArticle
dc.contributor.departmentINSTITUTE OF MOLECULAR & CELL BIOLOGY
dc.description.doi10.1007/BF00023395
dc.description.sourcetitlePlant Molecular Biology
dc.description.volume19
dc.description.issue3
dc.description.page473-484
dc.description.codenPMBID
dc.identifier.isiutA1992JA98800013
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