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|Title:||Thermus aquaticus DNA polymerase-catalysed chain reaction for the detection of human papillomaviruses||Authors:||Chow, V.T.K.
|Keywords:||Human papillomavirus (HPV)
Polymerase chain reaction (PCR)
Thermus aquaticus DNA polymerase
|Issue Date:||Jan-1990||Citation:||Chow, V.T.K.,Tham, K.M.,Bernard, H.U. (1990-01). Thermus aquaticus DNA polymerase-catalysed chain reaction for the detection of human papillomaviruses. Journal of Virological Methods 27 (1) : 101-112. ScholarBank@NUS Repository.||Abstract:||To delineate the conditions for the polymerase chain reaction (PCR) using primers specific for human papillomavirus (HPV) types 6b, 16 and 18, a number of important technical features were analysed. Buffer, concentrations of magnesium, Taq polymerase, primers and DNA templates, annealing temperature, and extension time were studied by a combination of gel electrophoresis, Southern and slot-blot hybridization. Amplification of E6 gene fragments of HPV-16 and HPV-18 generated bands of 110 bp and 154 bp respectively, as predicted. However, amplification of a segment within the long control region of HPV 6b yielded an unexpected size of 340 bp. Different conditions were found for each HPV type-specific primer pair. These results, and the applications of PCR in HPV research and in an increasingly wide range of fields in medical virology are discussed. © 1990.||Source Title:||Journal of Virological Methods||URI:||http://scholarbank.nus.edu.sg/handle/10635/112138||ISSN:||01660934|
|Appears in Collections:||Staff Publications|
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