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Title: The human neuronal α1-chimaerin gene contains a position-dependent negative regulatory element in the first exon
Authors: Dong, J.-M. 
Lim, L.
Keywords: α1-Chimaerin
CAT assay
negative regulatory element
Issue Date: Sep-1996
Citation: Dong, J.-M., Lim, L. (1996-09). The human neuronal α1-chimaerin gene contains a position-dependent negative regulatory element in the first exon. Neurochemical Research 21 (9) : 1023-1030. ScholarBank@NUS Repository.
Abstract: α1-Chimaerin mRNA, which encodes a neuron-specific GTPase-activating protein for the signal transduction molecule p21 Rac, is highly expressed in certain brain regions and neuronal cell lines. The promoter region of human α1-chimaerin transcriptional unit contains no TATA box, Sp1-binding site or initiator motif. However, a CCAAT box located in the proximal promoter region is essential for promoter activity. We now describe a negative regulatory element in the 5' untranslated region of exon 1 of the human α1-chimaerin gene. Deletion of this 70-bp region from the α1-chimaerin minimal promoter increased the promoter activity 5- to 6-fold. The negative element can suppress heterologous thymidine kinase promoter activity in an orientation- independent manner when placed in its native position. However, its function is position-dependent. The presence of a putative factor in rat liver, HepG2 and SK-N-SH cell nuclear extracts but not in rat brain nuclear extract which interacts with this element suggests a possible role of the negative element in controlling the neuron-specific expression of α1-chimaerin in vivo.
Source Title: Neurochemical Research
ISSN: 03643190
DOI: 10.1007/BF02532412
Appears in Collections:Staff Publications

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