Please use this identifier to cite or link to this item: https://doi.org/10.1093/nar/gkn414
Title: TAp73β and DNp73β activate the expression of the pro-survival caspase-2S
Authors: Toh, W.H.
Logette, E.
Corcos, L.
Sabapathy, K. 
Issue Date: Aug-2008
Citation: Toh, W.H., Logette, E., Corcos, L., Sabapathy, K. (2008-08). TAp73β and DNp73β activate the expression of the pro-survival caspase-2S. Nucleic Acids Research 36 (13) : 4498-4509. ScholarBank@NUS Repository. https://doi.org/10.1093/nar/gkn414
Abstract: p73, the p53 homologue, exists as a transactivation-domain-proficient TAp73 or deficient deltaN(DN)p73 form. Expectedly, the oncogenic DNp73 that is capable of inactivating both TAp73 and p53 function, is over-expressed in cancers. However, the role of TAp73, which exhibits tumour-suppressive properties in gain or loss of function models, in human cancers where it is hyper-expressed is unclear. We demonstrate here that both TAp73 and DNp73 are able to specifically transactivate the expression of the anti-apoptotic member of the caspase family, caspase-2S. Neither p53 nor TAp63 has this property, and only the p73β form, but not the p73α form, has this competency. Caspase-2 promoter analysis revealed that a non-canonical, 18 bp GC-rich Sp-1-binding site-containing region is essential for p73α-mediated activation. However, mutating the Sp-1-binding site or silencing Sp-1 expression did not affect p73β's transactivation ability. In vitro DNA binding and in vivo chromatin immunoprecipitation assays indicated that p73β is capable of directly binding to this region, and consistently, DNA binding p73 mutant was unable to transactivate caspase-2S. Finally, DNp73β over-expression in neuroblastoma cells led to resistance to cell death, and concomitantly to elevated levels of caspase-2S. Silencing p73 expression in these cells led to reduction of caspase-2S expression and increased cell death. Together, the data identifies caspase-2S as a novel transcriptional target common to both TAp73 and DNp73, and raises the possibility that TAp73 may be over-expressed in cancers to promote survival. © 2008 The Author(s).
Source Title: Nucleic Acids Research
URI: http://scholarbank.nus.edu.sg/handle/10635/112099
ISSN: 03051048
DOI: 10.1093/nar/gkn414
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