Please use this identifier to cite or link to this item: https://doi.org/10.1007/s002990050099
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dc.titleSynergistic effect of ethylene inhibitors and putrescine on shoot regeneration from hypocotyl explants of Chinese radish (Raphanus sativus L. var. longipinnatus Bailey) in vitro
dc.contributor.authorPua, E.-C.
dc.contributor.authorSim, G.-E.
dc.contributor.authorChi, G.-L.
dc.contributor.authorKong, L.-F.
dc.date.accessioned2014-11-28T02:53:09Z
dc.date.available2014-11-28T02:53:09Z
dc.date.issued1996
dc.identifier.citationPua, E.-C.,Sim, G.-E.,Chi, G.-L.,Kong, L.-F. (1996). Synergistic effect of ethylene inhibitors and putrescine on shoot regeneration from hypocotyl explants of Chinese radish (Raphanus sativus L. var. longipinnatus Bailey) in vitro. Plant Cell Reports 15 (9) : 685-690. ScholarBank@NUS Repository. <a href="https://doi.org/10.1007/s002990050099" target="_blank">https://doi.org/10.1007/s002990050099</a>
dc.identifier.issn07217714
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/112097
dc.description.abstractThe role of ethylene and putrescine on shoot regeneration from hypocotyl explants of Chinese radish (Raphanus sativus L. var. longipinnatus Bailey cv. Red Coat) was investigated. Explants were recalcitrant in culture, but exogenous application of ethylene inhibitor 120-30 μM aminoethoxyvinylglycine (AVG) or AgNO3] enhanced shoot regeneration of explants grown on medium supplemented with 2 mg/l N6-benzyladenine and 1 mg/l 1-naphthaleneacetic acid. The best regeneration occurred in the medium containing AgNO3 in combination with AVG. Culture medium solidified with agarose in the presence of AgNO3 but not AVG was also beneficial to shoot regeneration. Exogenous putrescine, 2-chloroethylphosphonic acid and 1- aminocyclopropane 1-carboxylate had no effect on shoot regeneration. However, regeneration was greatly promoted by 10-25 mM putrescine in combination with 30 μM AgNO3 or AVG. Explants with high regenerability grown in the presence of AgNO3 or in combination with putrescine emanated high levels of ethylene throughout the 21-d culture period. By contrast, AVG or putrescine alone resulted in a decrease in ethylene production. For rooting of shoot cuttings, IAA and IBA at 1-5 mg/l were more effective than NAA.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/s002990050099
dc.sourceScopus
dc.subjectAgNO3
dc.subjectaminoethoxyvinylglycine
dc.subjectChinese radish
dc.subjectethylene
dc.subjectputrescine
dc.subjectRaphanus sativus var. longipinnatus
dc.subjectshoot regeneration
dc.typeArticle
dc.contributor.departmentBOTANY
dc.contributor.departmentINSTITUTE OF MOLECULAR & CELL BIOLOGY
dc.description.doi10.1007/s002990050099
dc.description.sourcetitlePlant Cell Reports
dc.description.volume15
dc.description.issue9
dc.description.page685-690
dc.description.codenPCRPD
dc.identifier.isiutNOT_IN_WOS
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