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Title: Structure-related properties of the mutagenic lesion 6-O-methylguanine in DNA
Authors: Wong, C.-W. 
Tan, N.-W. 
Li, B.F.L. 
Keywords: 6-O-methylguanine
DNA structure
Human DNA (cytosine-5-)methyltransferase
Human methylguanine methyltransferase
Restriction enzyme MaeII
Issue Date: 1992
Citation: Wong, C.-W.,Tan, N.-W.,Li, B.F.L. (1992). Structure-related properties of the mutagenic lesion 6-O-methylguanine in DNA. Journal of Molecular Biology 228 (4) : 1137-1146. ScholarBank@NUS Repository.
Abstract: Chemical probing of the structures of a few very similar 30 base-pair duplexes containing a 6-O-methylguanine (meG) residue at the 16th position reveals that the modified base simultaneously perturbs the helical structure in two ways; it preferentially unstacks the 3′ neighbouring base residue (thymine in this study) on the same strand and it unstacks the pyrimidine to which it is base-paired. Depending on its neighbouring 5′ base residue and the base-pairing pyrimidine, this perturbation can extend to a few base-pairs in both 3′ and 5′ directions from the abnormal base-pair. These perturbations can be detected by cleavage at the site for the restriction enzyme MaeII. The unstaking of the C in the meG·C and A·C base-pairs may explain the de novo methylation of these helices by the human DNA-(cytosine-5-)methyltransferase. Interestingly, the kinetics of repair of the 6-O-methylguanine-containing dinucleotides by the cloned human methylguanine methyltransferase appears to be largely determined by the strength of the stacking interaction between the 6-O-methylguanine and the 5′ neighbouring base. © 1992 Academic Press Limited.
Source Title: Journal of Molecular Biology
ISSN: 00222836
Appears in Collections:Staff Publications

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