Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/112057
DC Field | Value | |
---|---|---|
dc.title | RNA slot blotting. | |
dc.contributor.author | Murphy, D. | |
dc.date.accessioned | 2014-11-28T02:52:42Z | |
dc.date.available | 2014-11-28T02:52:42Z | |
dc.date.issued | 1993 | |
dc.identifier.citation | Murphy, D. (1993). RNA slot blotting.. Methods in molecular biology (Clifton, N.J.) 18 : 341-343. ScholarBank@NUS Repository. | |
dc.identifier.issn | 19406029 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/112057 | |
dc.description.abstract | Using slot blots, RNA can be applied, unfractionated, to a solid matrix. Slot blotting can be used as a rapid method for analyzing changes in transgene RNA quantity following developmental or physiological changes. However, it can only be applied to animals that bear a transgene that has hybridizable segments with little or no homology to host RNAs expressed in the same tissues being examined-for example, RNAs derived from a hybrid gene with a viral or prokaryotic reporter element or a gene from another species with sufficient sequence divergence to allow the transgene RNAs to be distinguished from the host RNAs. | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | INSTITUTE OF MOLECULAR & CELL BIOLOGY | |
dc.description.sourcetitle | Methods in molecular biology (Clifton, N.J.) | |
dc.description.volume | 18 | |
dc.description.page | 341-343 | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Staff Publications |
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