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|Title:||RNA slot blotting.||Authors:||Murphy, D.||Issue Date:||1993||Citation:||Murphy, D. (1993). RNA slot blotting.. Methods in molecular biology (Clifton, N.J.) 18 : 341-343. ScholarBank@NUS Repository.||Abstract:||Using slot blots, RNA can be applied, unfractionated, to a solid matrix. Slot blotting can be used as a rapid method for analyzing changes in transgene RNA quantity following developmental or physiological changes. However, it can only be applied to animals that bear a transgene that has hybridizable segments with little or no homology to host RNAs expressed in the same tissues being examined-for example, RNAs derived from a hybrid gene with a viral or prokaryotic reporter element or a gene from another species with sufficient sequence divergence to allow the transgene RNAs to be distinguished from the host RNAs.||Source Title:||Methods in molecular biology (Clifton, N.J.)||URI:||http://scholarbank.nus.edu.sg/handle/10635/112057||ISSN:||19406029|
|Appears in Collections:||Staff Publications|
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