RNA slot blotting.

Abstract

Using slot blots, RNA can be applied, unfractionated, to a solid matrix. Slot blotting can be used as a rapid method for analyzing changes in transgene RNA quantity following developmental or physiological changes. However, it can only be applied to animals that bear a transgene that has hybridizable segments with little or no homology to host RNAs expressed in the same tissues being examined-for example, RNAs derived from a hybrid gene with a viral or prokaryotic reporter element or a gene from another species with sufficient sequence divergence to allow the transgene RNAs to be distinguished from the host RNAs.