Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/112036
Title: Random primed labeling of DNA.
Authors: Smith, D.R. 
Issue Date: 1993
Citation: Smith, D.R. (1993). Random primed labeling of DNA.. Methods in molecular biology (Clifton, N.J.) 18 : 445-447. ScholarBank@NUS Repository.
Abstract: Random primed labeling of DNA has now almost superseded the method of nick translation of DNA. Random primed labeling, based on the method of Feinberg and Vogelstein (1) is a method of incorporating radioactive nucleotides along the length of a fragment of DNA. Random primed labeling can give specific activities of between 2 × 10(9) and 5 × 10(9) dpm/μg (see Note 1). The following method is essentially that described by Feinberg and Vogelstein (2) in which a DNA fragment is denatured by heating in a boiling water bath. Then, random sequence oligonucleotides are annealed to both strands. Klenow fragment polymerase is then used to extend the oligonucleotides, using three cold nucleotides and one radioactively labeled nucleotide provided in the reaction mixture, to produce a uniformly labeled double-stranded probe. Each batch of random oligonucleotides contains all possible sequences (for hexamers, which are most commonly employed, this would be 4096 different oligonucleotides) and so any DNA template can be used with this method.
Source Title: Methods in molecular biology (Clifton, N.J.)
URI: http://scholarbank.nus.edu.sg/handle/10635/112036
ISSN: 19406029
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

100
checked on Feb 2, 2023

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.