Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/112021
Title: Primer extension.
Authors: Smith, D.R. 
Issue Date: 1993
Citation: Smith, D.R. (1993). Primer extension.. Methods in molecular biology (Clifton, N.J.) 18 : 373-378. ScholarBank@NUS Repository.
Abstract: Primer extension can be viewed as a technique complementary to S1 nuclease mapping (see Chapter 43). In Sl nuclease mapping analysis, a DNA:RNA hybrid is resected back to the point of divergence between the RNA and the DNA. In primer extension, a DNA probe is annealed to an RNA template and then extended in a 3';- 5'; direction to the start of the RNA molecule. The most common usages for this technique are in determining the size of full-length RNA transcripts and mapping transcription initiation sites.
Source Title: Methods in molecular biology (Clifton, N.J.)
URI: http://scholarbank.nus.edu.sg/handle/10635/112021
ISSN: 19406029
Appears in Collections:Staff Publications

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