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|Title:||Primer extension.||Authors:||Smith, D.R.||Issue Date:||1993||Citation:||Smith, D.R. (1993). Primer extension.. Methods in molecular biology (Clifton, N.J.) 18 : 373-378. ScholarBank@NUS Repository.||Abstract:||Primer extension can be viewed as a technique complementary to S1 nuclease mapping (see Chapter 43). In Sl nuclease mapping analysis, a DNA:RNA hybrid is resected back to the point of divergence between the RNA and the DNA. In primer extension, a DNA probe is annealed to an RNA template and then extended in a 3';- 5'; direction to the start of the RNA molecule. The most common usages for this technique are in determining the size of full-length RNA transcripts and mapping transcription initiation sites.||Source Title:||Methods in molecular biology (Clifton, N.J.)||URI:||http://scholarbank.nus.edu.sg/handle/10635/112021||ISSN:||19406029|
|Appears in Collections:||Staff Publications|
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