Please use this identifier to cite or link to this item:
Title: Primer extension.
Authors: Smith, D.R. 
Issue Date: 1993
Citation: Smith, D.R. (1993). Primer extension.. Methods in molecular biology (Clifton, N.J.) 18 : 373-378. ScholarBank@NUS Repository.
Abstract: Primer extension can be viewed as a technique complementary to S1 nuclease mapping (see Chapter 43). In Sl nuclease mapping analysis, a DNA:RNA hybrid is resected back to the point of divergence between the RNA and the DNA. In primer extension, a DNA probe is annealed to an RNA template and then extended in a 3';- 5'; direction to the start of the RNA molecule. The most common usages for this technique are in determining the size of full-length RNA transcripts and mapping transcription initiation sites.
Source Title: Methods in molecular biology (Clifton, N.J.)
ISSN: 19406029
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

checked on Jan 26, 2023

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.