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Title: Partial nucleotide sequence and organisation of extrachromosomal plastid-like DNA in Plasmodium berghei
Authors: Yap, M.W.C.
Kara, U.A.K.
Ten Heggeler-Bordier, B.
Ting, R.C.Y. 
Tan, T.M.C. 
Keywords: Extrachromosomal DNA
Plasmodium berghei
Issue Date: 24-Oct-1997
Citation: Yap, M.W.C., Kara, U.A.K., Ten Heggeler-Bordier, B., Ting, R.C.Y., Tan, T.M.C. (1997-10-24). Partial nucleotide sequence and organisation of extrachromosomal plastid-like DNA in Plasmodium berghei. Gene 200 (1-2) : 91-98. ScholarBank@NUS Repository.
Abstract: The murine malaria parasite Plasmodium berghei contains a plastid-like extrachromosomal genome. This genome is 30.7 kb in size and is transcriptionally active as shown by RT-PCR. DNA sequence analysis of the genome reveals 69.9-95.5% homology to sequences of the 35-kb extrachromosomal circle found in the human malaria species Plasmodium falciparum. Homologous sequences include regions of genes for the ssu-rRNA, lsu-rRNA, rpo B and clusters of t-RNAs. Sequence variation between the two Plasmodium species exists in the non-coding interspacing regions. A physical map has been constructed for the P. berghei circle, indicating the EcoRI and HindIII restriction sites as well as the arrangement of the rRNA, rpo B and tRNA genes. Arrangement of these genes is similar to that found on the P. falciparum 35-kb circle. The P. berghei circular element is distinct from the mitochondrial 6-kb DNA of both the murine and the human Plasmodium species. Preliminary results indicate that the circle may be a useful target for drug therapy.
Source Title: Gene
ISSN: 03781119
DOI: 10.1016/S0378-1119(97)00385-5
Appears in Collections:Staff Publications

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