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|Title:||Molecular and genetic characterization of SLC1, a putative Saccharomyces cerevisiae homolog of the metazoan cytoplasmic dynein light chain 1||Authors:||Dick, T.
|Issue Date:||1996||Citation:||Dick, T.,Surana, U.,Chia, W. (1996). Molecular and genetic characterization of SLC1, a putative Saccharomyces cerevisiae homolog of the metazoan cytoplasmic dynein light chain 1. Molecular and General Genetics 251 (1) : 38-43. ScholarBank@NUS Repository.||Abstract:||Cytoplasmic dynein is a multisubunit, microtubule-dependent motor enzyme that has been proposed to function in a variety of intracellular movements. As part of an effort to understand the evolution and the biological roles of cytoplasmic dynein, we have identified the first non-metazoan dynein light chain 1, SLC1, in the yeast Saccharomyces cerevisiae. The amino acid sequence of the SLC1 protein is similar to those of the human, Drosophila and Caenorhahditis cytoplasmic dynein light chains 1. The SLC1 gene lies adjacent to the YAP2 ( =CAD1) transcription unit. The 5LC1 coding sequence is split by two introns and its mRNA is detectable throughout the cell cycle. Tetrad analysis of heterozygotes harboring a TRP insertion in the SLC1 coding region indicate that SLC1 function is not essential for cell viability. Furthermore, we demonstrate that double mutants, defective for SLC1 and the kinesin-related CIN8 genes are non-lethal. The redundancy of SLC1 function in yeast contrasts with the cell death caused by loss-of-function mutations in the dynein light chain 1 gene in Drosophila melanoaaster.||Source Title:||Molecular and General Genetics||URI:||http://scholarbank.nus.edu.sg/handle/10635/111973||ISSN:||00268925|
|Appears in Collections:||Staff Publications|
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