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|Title:||K-ras mutation in colorectal carcinoma from Singapore||Authors:||Phang, T.-W.
|Issue Date:||1995||Citation:||Phang, T.-W.,Shi, C.-Y.,Wee, A.,Ngoi, S.-S.,Li, B.,Lee, H.-P.,Ong, C.-N. (1995). K-ras mutation in colorectal carcinoma from Singapore. International Journal of Oncology 6 (1) : 191-195. ScholarBank@NUS Repository.||Abstract:||54 sporadic colorectal cancers were analyzed for aberrations in the K-ras oncogene. DNA was extracted from frozen tissues obtained from surgical resection and analyzed for mutations in codons 12, 13 and 61 of the K-ras oncogene using single strand conformation polymorphism analysis (SSCP) and direct sequencing. Point mutations in the K-ras oncogene were found in 26/54 (48%) cases, all of which resulted in amino acid substitutions. No other types of mutations (e.g. insertions or deletions) were found. 4 of the mutations were at codon 12, 21 in codon 13 and only 1 was a codon 61 mutant. G→A transitions were found to be predominant. A remarkable finding was the high preponderance of 13Gly→13Ser mutations (54%). No correlation was observed between K-ras mutations and tumor location, Dukes' stage, differentiation levels, age or sex of the patient.||Source Title:||International Journal of Oncology||URI:||http://scholarbank.nus.edu.sg/handle/10635/111957||ISSN:||10196439|
|Appears in Collections:||Staff Publications|
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