Please use this identifier to cite or link to this item: https://doi.org/10.1007/BF00039734
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dc.titleHigh frequency of plant regeneration from hypocotyl explants of Brassica carinata A. Br.
dc.contributor.authorYang, M.-Z.
dc.contributor.authorJia, S.-R.
dc.contributor.authorPua, E.-C.
dc.date.accessioned2014-11-28T02:51:05Z
dc.date.available2014-11-28T02:51:05Z
dc.date.issued1991-02
dc.identifier.citationYang, M.-Z., Jia, S.-R., Pua, E.-C. (1991-02). High frequency of plant regeneration from hypocotyl explants of Brassica carinata A. Br.. Plant Cell, Tissue and Organ Culture 24 (2) : 79-82. ScholarBank@NUS Repository. https://doi.org/10.1007/BF00039734
dc.identifier.issn01676857
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/111913
dc.description.abstractAn efficient tissue culture system for high frequency of plant regeneration from hypocotyl explants of Brassica carinata was developed via manipulation of culture medium and selection of explants. Explants grown on medium containing combinations of 2 mg l-1 BA and 0.01 mg l-1 NAA or 4 mg l-1 kinetin and 0.01 mg l-1 2,4-D regenerated shoots at 100% frequency. High frequency shoot regeneration occurred only from explants originating from 6 to 7-day-old but not younger or older seedlings. Explants showed higher regeneration capacity at the distal end than the proximal end, and the upper segment was more regenerative than the lower segment of hypocotyl. Regenerants were rooted on half-strength growth regulator-free medium, acclimatized and developed into normal, fertile plants. © 1991 Kluwer Academic Publishers.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/BF00039734
dc.sourceScopus
dc.subjectBrassica carinata
dc.subjectEthiopia mustard
dc.subjecthypocotyl
dc.subjectplant regeneration
dc.subjecttissue culture
dc.typeArticle
dc.contributor.departmentINSTITUTE OF MOLECULAR & CELL BIOLOGY
dc.description.doi10.1007/BF00039734
dc.description.sourcetitlePlant Cell, Tissue and Organ Culture
dc.description.volume24
dc.description.issue2
dc.description.page79-82
dc.description.codenPTCED
dc.identifier.isiutA1991FB38400003
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