Please use this identifier to cite or link to this item: https://doi.org/10.1007/BF00039734
Title: High frequency of plant regeneration from hypocotyl explants of Brassica carinata A. Br.
Authors: Yang, M.-Z.
Jia, S.-R.
Pua, E.-C. 
Keywords: Brassica carinata
Ethiopia mustard
hypocotyl
plant regeneration
tissue culture
Issue Date: Feb-1991
Citation: Yang, M.-Z., Jia, S.-R., Pua, E.-C. (1991-02). High frequency of plant regeneration from hypocotyl explants of Brassica carinata A. Br.. Plant Cell, Tissue and Organ Culture 24 (2) : 79-82. ScholarBank@NUS Repository. https://doi.org/10.1007/BF00039734
Abstract: An efficient tissue culture system for high frequency of plant regeneration from hypocotyl explants of Brassica carinata was developed via manipulation of culture medium and selection of explants. Explants grown on medium containing combinations of 2 mg l-1 BA and 0.01 mg l-1 NAA or 4 mg l-1 kinetin and 0.01 mg l-1 2,4-D regenerated shoots at 100% frequency. High frequency shoot regeneration occurred only from explants originating from 6 to 7-day-old but not younger or older seedlings. Explants showed higher regeneration capacity at the distal end than the proximal end, and the upper segment was more regenerative than the lower segment of hypocotyl. Regenerants were rooted on half-strength growth regulator-free medium, acclimatized and developed into normal, fertile plants. © 1991 Kluwer Academic Publishers.
Source Title: Plant Cell, Tissue and Organ Culture
URI: http://scholarbank.nus.edu.sg/handle/10635/111913
ISSN: 01676857
DOI: 10.1007/BF00039734
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