Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/111869
Title: Efficient synthesis of mosquitocidal toxins in Asticcacaulis excentricus demonstrates potential of gram-negative bacteria in mosquito control
Authors: Liu, J.-W. 
Yap, W.H. 
Thanabalu, T. 
Porter, A.G. 
Keywords: Asticcacaulis excentricus
insecticide
Issue Date: 1996
Citation: Liu, J.-W.,Yap, W.H.,Thanabalu, T.,Porter, A.G. (1996). Efficient synthesis of mosquitocidal toxins in Asticcacaulis excentricus demonstrates potential of gram-negative bacteria in mosquito control. Nature Biotechnology 14 (3) : 343-347. ScholarBank@NUS Repository.
Abstract: The control of mosquitoes with chemical insecticides pollutes the environment and leads to resistance in mosquito populations. Bacterial control of mosquito larvae with Bacillus sphaericus and Bacillus thuringiensis subsp. israelensis, which produce protein toxins, has proved useful, safe, and nonpolluting. These bacteria do, however, suffer from disadvantages, including rapid settling, UV sensitivity, and lack of persistance of spores, proteolysis of toxins, narrow host range, and high production costs. Here we show that the Gram-negative bacterium Asticcacaulis excentricus is a promising host for delivering toxins to mosquito larvae. Plasmid-transformed A. excentricus cells expressing the binary toxin of B. sphaericus exhibited toxicity to Culex and Anopheles mosquito larvae similar to that of the high-toxicity strains of B. sphaericus which produce several toxins. A. excentricus has potential advantages as a larvicide compared with the bacilli, especially persistance in the larval feeding zone, resistance to UV light, lack of toxin-degrading proteases, and low production costs.
Source Title: Nature Biotechnology
URI: http://scholarbank.nus.edu.sg/handle/10635/111869
ISSN: 0733222X
Appears in Collections:Staff Publications

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