Please use this identifier to cite or link to this item:
|dc.title||Differential effect of Staphylococcal enterotoxin B upon the induction of tolerance on peripheral CD4+Vβ8+ and CD8+Vβ8+ T cells|
|dc.identifier.citation||Sabapathy, T.K., Hwang, L.A., Hui, K.M. (1994). Differential effect of Staphylococcal enterotoxin B upon the induction of tolerance on peripheral CD4+Vβ8+ and CD8+Vβ8+ T cells. Cellular Immunology 158 (1) : 83-95. ScholarBank@NUS Repository. https://doi.org/10.1006/cimm.1994.1258|
|dc.description.abstract||It is known that bacterial superantigens can interact with certain VP elements of the T cell receptor to result in the activation, expansion, anergy, and/or deletion of T cells. The induction of peripheral T cell tolerance in AKR/J mice was examined in relation to the amount of staphylococcal enterotoxin B (SEB) administered and it was found that the events leading to the induction of tolerance of Vβ8+ T cells was dependent on the initial dose of superantigen employed. Following administration of a large amount (≥ 10 μg) of SEB into AKR/J mice, expansion of both CD4+Vβ8+ and CD8+Vβ8+ T cells was observed. This initial cell expansion was followed by the decline in the number of CD4+Vβ8+ T cells. The number of CD8+Vβ8+ T cells, however, did not decline and remained high. When a small amount (2 μg) of SEB was employed, it did not stimulate T cell expansion in AKR/J mice. However, when these mice were challenged with SEB, anergy was observed in the CD4+Vβ8+ T cells regardless of the initial dose of SEB. In contrast, the CD8+Vβ8+ T cells were not anergized and were able to proliferate on stimulation with a second dose of SEB. The state of anergy for the CD4+Vβ8+ T cells lasted for at least 70 days, and by 150 days the anergic state was relieved and these CD4+VP8+ T cells were once again able to proliferate in response to SEB. On the other hand, continuous SEB exposure resulted in the decline of both CD4+VP8+ and CD8+Vβ8+ T cells. Although the number of CD4+Vβ8+ and CD8+Vβ8+ T cells apparently returned to normal levels by 150 days, the state of anergy persisted, as demonstrated by the reduction of the response of these T cells following SEB stimulation in vitro. Our data suggest that the initial expansion of T cells is not an absolute prerequisite for the induction of peripheral T cell anergy. Moreover, the continuous presence of superantigen is essential for the deletion and maintenance of a state of anergy for CD8+Vβ+ T cells.|
|dc.contributor.department||INSTITUTE OF MOLECULAR & CELL BIOLOGY|
|Appears in Collections:||Staff Publications|
Show simple item record
Files in This Item:
There are no files associated with this item.
checked on Apr 30, 2021
WEB OF SCIENCETM
checked on Apr 30, 2021
checked on May 2, 2021
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.